Circular RNAs

The landscape of RNA biology is evolving. Beyond the well-trodden path of linear RNAs, a new class of molecules, circular RNAs (circRNAs), has emerged as a major force in gene regulation. These covalently closed RNA loops exhibit remarkable stability due to their inherent resistance to exonuclease degradation. This stability, combined with their diverse functions as miRNA sponges, protein scaffolds, and even templates for translation, makes them exceptionally promising. Once dismissed as transcriptional byproducts, circRNAs are now at the forefront of research into cancer, neurobiology, and regenerative medicine, opening up unprecedented opportunities for novel diagnostics and therapeutics.

Fig.1 Applications of circRNAs. (OA Literature) Fig.1 Application of engineered circRNAs.^1,2

Technology Spotlight: Next-Generation circRNA Overexpression Vectors

Experience a paradigm shift in circRNA research with our upgraded overexpression vectors. Available in both plasmid and lentiviral formats, this system is engineered for superior performance, achieving up to 1000-fold overexpression and an outstanding circularization efficiency of over 95%.

Unprecedented Overexpression Efficiency: Our vectors feature a patented flanking circularization framework. This proprietary technology has been rigorously validated to ensure significant and reliable overexpression for all tested circRNAs.
Perfect Circularization Fidelity: Embedded within the framework are proprietary circularization-mediating elements that guarantee the precise back-splicing of your target linear sequence. All validated circRNAs are circularized with perfect fidelity, with no base additions or deletions.
Robust and Versatile Performance: Our system demonstrates high stability and efficiency across a wide range of insert sizes. We have successfully achieved accurate and efficient overexpression for target circRNAs ranging from 200bp to 2500bp.
Broad Applicability: The vector backbones are designed for maximum flexibility, suitable for standard eukaryotic expression, high-titer lentivirus packaging, and easy screening with integrated green fluorescence (GFP) and puromycin resistance markers.

Our Comprehensive circRNA Portfolio

To meet the diverse needs of the scientific community, we offer a portfolio that spans fully customized synthesis to an extensive catalog of ready-to-use products.

1. Custom circRNA Development Services:

Custom IVT Circular RNA Synthesis: We provide high-purity, sequence-verified circRNAs synthesized to your exact specifications. Our optimized in vitro transcription and proprietary circularization technology guarantee high yields and structural integrity, perfect for functional studies.
Custom LNP-encapsulated Circular RNA: Bridge the gap between in vitro discovery and in vivo application with our cutting-edge Lipid Nanoparticle (LNP) delivery platform. We encapsulate your custom circRNA in tailored LNP formulations, ensuring enhanced stability, efficient cellular uptake, and effective delivery for pre-clinical and therapeutic research.

2. Pre-made, Ready-to-Use circRNAs Catalog:

Accelerate your research with our vast collection of off-the-shelf, validated circRNAs. Our products are meticulously designed and produced, covering a wide array of research areas:

Cancer Research & Immunotherapy: circRNAs encoding Tumor Antigens, Anti-tumor factors, and potent Immunotherapy modulators.
Regenerative Medicine & Anti-aging: A powerful selection including Growth Factors, iPSC Reprogramming factors, Anti-aging agents, Neurotrophic Factors, and Neural Regeneration/Conversion factors.
Biotech Tools & Gene Editing: Versatile tools such as circRNAs carrying Reporter Genes (e.g., Luciferase), components for Gene Editing systems, and constructs for Protein/Antibody Replacement.
Endogenous Studies: A wide range of well-characterized, Natural Circular RNAs to investigate their native biological functions.

Our circRNA products and services are engineered to drive innovation across critical applications. With our custom synthesis and LNP delivery platforms, you can develop next-generation therapeutics for targeted cancer therapy or neurodegenerative diseases. Our pre-made circRNAs encoding growth factors or tumor antigens provide a direct path for pre-clinical investigation. Furthermore, the exceptional stability of circRNAs makes them ideal for developing non-invasive liquid biopsy biomarkers, and our research tools are essential for dissecting their fundamental roles in cellular and disease biology.

Ready to harness the immense potential of circular RNA? Browse our extensive catalog of pre-made products or consult our experts to design a custom solution for your project. Let's pioneer the future of RNA science, together.

Why Partner with Creative Biolabs?

Creative Biolabs is your dedicated scientific partner in navigating the exciting world of circular RNAs. We go beyond simply supplying products; we provide a holistic, end-to-end research solution designed to guide your project from initial hypothesis to publication-ready data. Our expert team supports you through every stage of the research pipeline.

Our Custom circRNA Synthesis Workflow:

Fig.2 Custom circRNA Synthesis Workflow. (Choi, 2024) (OA Literature)

Our integrated circRNA Total Research Solution:

Fig.3 circRNA Total Research Workflow. (Creative Biolabs Original)

References

Choi, Seo-Won, and Jin-Wu Nam. "Optimal design of synthetic circular RNAs." Experimental & molecular medicine vol. 56,6 (2024): 1281-1292. doi:10.1038/s12276-024-01251-w.
Distributed under Open Access License CC BY 4.0. The original image was modified.
Distributed under Open Access License CC BY 4.0 without modification.

For Research Use Only. Not For Clinical Use.

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