GABA_A Receptor α1 Knockout Mice Development Service

Are you currently facing long drug development cycles or struggling with limited effective treatments for Essential tremor (ET)? Our advanced preclinical services at Creative Biolabs help you accelerate drug discovery and gain a deeper understanding of ET pathophysiology through our innovative GABA_A receptor alpha1 (α1) subunit knockout mouse model.

ET is the most common movement disorder, characterized by involuntary rhythmic shaking. Its precise cause remains elusive, leading to a significant unmet need for effective treatments. The GABA_A receptor α1 subunit knockout mouse model has emerged as a crucial tool, offering unprecedented insights into ET's underlying mechanisms. This model faithfully mimics human ET symptoms, providing a robust platform for identifying novel therapeutic targets and accelerating the development of much-needed pharmacological interventions.

How Our GABA_A Receptor α1 Subunit Knockout Mice Can Assist Your Project

At CBL, we provide comprehensive solutions for advancing your ET research and drug development. Our GABA_A receptor α1 subunit knockout mouse model offers a robust platform for target validation, preclinical drug efficacy testing, and mechanistic studies, directly addressing the challenges of developing novel therapies for this debilitating movement disorder. We deliver precise, actionable data to guide your research decisions.

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Workflow

Our streamlined workflow ensures efficient and high-quality results for your ET research project.

• Required Starting Materials:

To initiate your project, clients typically provide:

1. Target Compound Information: Detailed chemical structures, preliminary in vitro data, and desired dosing regimens for your therapeutic candidates.
2. Specific Research Questions: Clearly defined objectives for the study, such as assessing tremor reduction, evaluating off-target effects, or exploring specific neurological pathways.
3. Project Scope and Timeline: Any specific requirements regarding the number of animals, duration of studies, or critical deadlines.

• Final Deliverables:

Upon project completion, you will receive:

1. Comprehensive Study Report: A detailed scientific report outlining methods, results, statistical analyses, and interpretation of findings.
2. Raw Data Files: All raw behavioral, electrophysiological, and biochemical data for your internal analysis and record-keeping.
3. Presentation of Key Findings: A summary presentation highlighting the most critical results and their implications for your drug development program.

• Estimated Timeframe:

The typical timeframe for studies utilizing the GABA_A receptor α1 subunit knockout mouse model ranges from 12 to 20 weeks, depending on the complexity of the experimental design, the number of compounds to be tested, and the depth of mechanistic investigations required.

Why Choose Us?

CBL stands at the forefront of neuroscience research, offering unparalleled expertise and a proven track record in utilizing the GABA_A receptor α1 subunit knockout mouse model for Essential Tremor research. Our commitment to scientific rigor, innovative approaches, and client success sets us apart.

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Customer Reviews:

"Exceptional Model Fidelity: Using CBL's ET GABA_A Receptor α1 Subunit Knockout Mice in our research has significantly improved the translational relevance of our findings. The model's accurate recapitulation of human ET symptoms allowed us to confidently assess our lead compounds, a critical step often missed with less specific models." [2 months ago], Jo***n S.

"Unmatched Mechanistic Insights: CBL's expertise in electrophysiology with the ET GABA_A Receptor α1 Subunit Knockout Mice facilitated unparalleled mechanistic insights into our compound's action on cerebellar circuits. This detailed data was instrumental in refining our drug's target profile and understanding its precise therapeutic effects." [1 month ago], M***a L.

"Streamlined Drug Screening: The efficiency of CBL's workflow for the ET GABA_A Receptor α1 Subunit Knockout Mice model allowed us to screen multiple compounds rapidly, identifying promising candidates that showed significant tremor reduction. Their comprehensive reports and responsive team made the entire process seamless and highly productive." [3 weeks ago], Al***x K.

GABA_A Receptor α1 Subunit Knockout Mice

The GABA_A receptor α1 subunit knockout (Gabra1 KO) mouse model is a cornerstone in ET research, offering a highly translatable platform to investigate disease pathophysiology and test novel therapeutics. This genetically engineered model, where the α1 subunit of the GABA_A receptor is specifically deleted, provides a powerful tool for understanding the complex interplay of GABAergic dysfunction in tremor genesis.

Fig.1 GABA_A receptor α1^–/– mice exhibit essential-like tremor.¹

Broader Neurological Phenotypes: Beyond tremor, Gabra1 KO mice exhibit a range of other neurological phenotypes, underscoring the broad physiological importance of the α1 subunit in central nervous system function. These include:

• Altered Anxiety Profiles: Gabra1 KO mice demonstrate heightened anxiety-like behaviors in various assays, such as decreased marble burying and reduced exploration of open, brightly lit areas.
• Sleep Disturbances: Detailed EEG recordings reveal significant alterations in sleep architecture, including increased non-rapid eye movement (NREM) sleep and decreased rapid eye movement (REM) sleep and waking time. Changes in specific EEG frequency bands (e.g., reduced delta, increased beta) further characterize these sleep abnormalities.
• Increased Seizure Susceptibility: The model also shows increased susceptibility to bicuculline-induced seizures, emphasizing the α1 subunit's role in regulating central nervous system (CNS) excitability.

What We Can Offer

At CBL, our ET GABA_A receptor α1 subunit knockout mice service is designed to provide comprehensive support and customized solutions for your preclinical research needs. We are dedicated to delivering high-quality, reliable data that accelerates your drug discovery pipeline.

• Customized Study Design: We offer tailored experimental protocols to precisely match your specific research objectives, compound characteristics, and desired outcomes for ET studies.
• Expert Animal Model Management: Our team ensures the highest standards of animal welfare, breeding, and genetic characterization, guaranteeing consistent and robust Gabra1 KO mouse cohorts for your studies.
• Advanced Phenotyping Capabilities: We provide a full suite of behavioral, electrophysiological, and biochemical assays to thoroughly characterize ET-like symptoms, anxiety, sleep disturbances, and the mechanistic effects of your compounds.
• Rigorous Quality Control: All data generated undergoes stringent quality control measures, ensuring accuracy, reproducibility, and scientific integrity, adhering to the highest industry standards.
• Comprehensive Data Analysis and Interpretation: Our experts provide in-depth analysis and insightful interpretation of your results, translating complex data into clear, actionable conclusions to guide your therapeutic development.
• Flexible Study Modes: We can accommodate various study designs, from initial screening to detailed mechanistic investigations, adapting to your project's evolving needs and timelines.
• Dedicated Scientific Support: You will have direct access to our experienced scientific team throughout your project, ensuring seamless communication and collaborative problem-solving.

Related Services

To further support your comprehensive drug discovery and development efforts in neurological disorders, CBL offers a suite of complementary services:

• Neuroinflammation Assays
• Neurodegenerative Disease Models
• Biomarker Discovery & Validation
• Histology & Pathology Services

Ready to unlock new therapeutic avenues for ET? Our team of experienced scientists is eager to discuss your project and demonstrate how our expertise and cutting-edge platforms can support your goals.

Contact Our Team for More Information and to Discuss Your Project.

Reference

1. Kralic, Jason E et al. "Genetic essential tremor in gamma-aminobutyric acidA receptor alpha1 subunit knockout mice." The Journal of clinical investigation vol. 115,3 (2005): 774-9. doi:10.1172/JCI23625. Distributed under Open Access license CC BY 4.0, without modification.