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Creative Biolabs
Product

NeuroMab™ Anti-Alpha Synuclein Antibody, Clone NR52P

[CAT#: NRP-0422-P637]

A functional antibody raised against Human Alpha Synuclein.

Host Species:
Rabbit
Species Reactivity:
Human
Applications:
DB; ELISA; FC; IHC; In Vitro; Cyt; In Vivo

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Product Overview

Description

This antibody has an alpha-synuclein aggregation dependent binding profile, i.e. this antibody binds to monomeric, dimeric and oligomeric human alpha-synuclein.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Rabbit

Clone Number

NR52P

Applications

DB; ELISA; FC; IHC; In Vitro; Cyt; In Vivo
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

Alpha Synuclein

Official Name

SNCA

Full Name

Alpha-synuclein

Alternative Names

SNCA; NACP; PARK1; PARK4; PD1; synuclein alpha

Gene ID

6622 (Human); 20617 (Mouse)

Uniprot ID

Product Pictures
IF

Figure 1 α-synuclein staining in the brainstem of α-synuclein [A30P] transgenic mice.

Fresh-frozen 10 μm sagittal brain section; 40X magnification; all images under the same lighting conditions; arrows indicate Lewy neurite-like inclusions.

IF

Figure 2 Staining of the cerebral cortex of patients with Parkinson's disease with antibodies 0017 and 0018.

(A), Alzheimer's disease patient with Parkinson's disease comorbidity (B) and progressive supranuclear palsy (PSP, tauopathy) (C); Arrowhead: Lewy body-like inclusion; Arrowhead: Lewy neurite- like inclusions.

Cyt

Figure 3 Cytotoxicity of conditioned medium of recombinant α-synuclein from SHSY5Y cells on LUHMES cells.

(A), Alzheimer's disease patient with Parkinson's disease comorbidity (B) and progressive supranuclear palsy (PSP, tauopathy) (C); Arrowhead: Lewy body-like inclusion; Arrowhead: Lewy neurite- like inclusions.

FuncS

Figure 4 The antibodies are capable of immunodepleting α-synuclein oligomers in the extracellular medium, thus these antibodies reduce the toxicity of α-synuclein oligomers.

(A), Alzheimer's disease patient with Parkinson's disease comorbidity (B) and progressive supranuclear palsy (PSP, tauopathy) (C); Arrowhead: Lewy body-like inclusion; Arrowhead: Lewy neurite- like inclusions.

FuncS

Figure 5. After acute peripheral injection of anti-α-synuclein-antibody-blood-brain barrier shuttle module conjugates, brain α-synuclein pathology and vasculature of α-synuclein mutant A30P transgenic mice and markers of parenchymal tissue.

(A), Alzheimer's disease patient with Parkinson's disease comorbidity (B) and progressive supranuclear palsy (PSP, tauopathy) (C); Arrowhead: Lewy body-like inclusion; Arrowhead: Lewy neurite- like inclusions.

IHC

Figure 6 Confocal microscopy analysis of immunohistochemical cryosections of brain sections from patients with Parkinson's disease (same settings for all images).

(A) Antibody 0017, (B) Antibody 0018.

SPR

Figure 7 Binding of monomeric (N-terminal free (1) and N-terminal His-tagged (2)) and dimeric (3) α-synuclein to antibody 0018.

The displayed result is a titration of 5 concentrations. 1: Monomer, free N-terminal, 4.2 mg/mL, never thawed; 2: Monomer, His-tagged N-terminal, 4.8 mg/mL; 3: Dimer, free N-terminal, 1.6 mg/mL.

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For Research Use Only. Not For Clinical Use.
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