NeuroMab™ Anti-GARP Antibody(NRP-0422-P1637)

Product Overview

Description

The NRP-0422-P1637 antibody directed against human GARP; inhibits active TGF-β production by human Treg cells. MHGARP8 does not bind mGARP. Its binding to hGARP requires a region comprising amino-acids 101 to 141.

Species Reactivity

Human; Cynomolgus Monkey

Clonality

Monoclonal

Host Species

Mouse

Applications

FC; Inhib; Block; In Vitro; In Vivo

Relevant Diseases

Amyotrophic Lateral Sclerosis

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

> 95% (SDS-PAGE)

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

GARP

Official Name

LRRC32

Full Name

Leucine Rich Repeat Containing 32

Alternative Names

Leucine Rich Repeat Containing 32; Glycoprotein A Repetitions Predominant; Transforming Growth Factor Beta Activator LRRC3; Leucine-Rich Repeat-Containing Protein 3; D11S833E; Garpin; GARP; LRRC32

Gene ID

2615(Human); 434215(Mouse)

Uniprot ID

Q14392(Human)

Product Pictures

Inhib

Figure 1 Inhibition of human Treg function by anti-hGARP in vivo.

Inhib

Figure 2 MHGARP8 inhibits production of active TGF-β by human Treg clones.

FCM

Figure 3 Insufficient presentation of hTGF-β1 on the cell surface for binding by MHGARP8. 293T cells were transfected as indicated below, stained with anti-hLAP antibody or MGARP8, and analyzed by flow cytometry.

FuncS

Figure 4 MHGARP8 inhibits Treg function in vivo. On day 0, designated groups of NSG mice received intravenous injections. Human PBMC were injected with or without human Tregs.