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Creative Biolabs
Product

NeuroMab™ Anti-Notch 1 Antibody, Clone NR158P

[CAT#: NRP-0422-P1181]

Host Species:
Mouse
Species Reactivity:
Human
Applications:
FC; In Vitro; Inhib

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Product Overview

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

NR158P

Applications

FC; In Vitro; Inhib

Relevant Diseases

Alzheimer's Disease; Epilepsy; Pain
Product Properties

Preservatives

BSA Free

Concentration

1mg/mL

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

Notch1

Official Name

NOTCH1

Full Name

NOTCH1

Alternative Names

Notch 1; Translocation-Associated Notch Protein TAN-1; TAN1; HN1; Notch (Drosophila) Homolog 1 (Translocation-Associated); Notch Homolog 1; Translocation-Associated (Drosophila); Notch Homolog 1; Translocation-Associated;

Gene ID

4851 (Human); 18128 (Mouse); 25496 (Rat)

Uniprot ID

P46531 (Human); Q01705 Mouse (Mouse); Q07008 Rat (Rat)
Product Pictures
FCM

Figure 1: Binding of anti-NOTCH1 monoclonal antibodies 13M57 and 31M80 to cell surface expressed NOTCH1.

FACS analysis of cells co-expressing full length NOTCH1 receptor and GFP incubated with, from left to right, IgG1 control antibodies, 13M57 and anti-human NOTCH1 EGF 1-5 antisera.

FuncS

Figure 10: Effect of combination therapy with anti-NOTCH1 antibody 13M57 and chemotherapy on growth of established colon tumors.

FCM

Figure 2: Binding of anti-NOTCH1 monoclonal antibodies 13M57 and 31M80 to cell surface expressed NOTCH1.

Anti-NOTCH1 antibodies 13M57 and 31M80 showed increased binding to PE13 breast tumor cells compared to an isotype control antibody (bottom) and this binding corresponded to cells that express high levels of ESA and CD44 (top: CD44 x-axis and NOTCH1 y-axis).

FuncS

Figure 3: Antibodies against NOTCH1 EGF13 and EGF4 do not effectively block ligand binding.

NOTCH1 expressing HEK 293 cells were incubated with either DLL4-Fc (left) or JAG1-Fc (right) in the presence of anti-NOTCH1 antibodies (13M57, 31M103, 31M106, or 31M108) or control anti-DLL4 (21M18) or anti-JAG1 (64M14) antibodies.

FuncS

Figure 4: Effect of NOTCH1 mAb against EGF4 on breast tumor cell growth in vitro.

Breast tumor cells were cultured for three days in the presence of 2.5 ug/mL or 5 ug/mL anti-NOTCH1 antibody, control mouse IgG, or no antibody, followed by 18 hr BrdU labeling.

FuncS

Figure 5: Effect of NOTCH1 monoclonal antibody 13M57 on PE-13 tumor cells in vivo.

NOD/SCID mice injected with PE-13 tumor cells were treated with PBS or anti-NOTCH1 antibodies 3 days after cell injection, and the growth of tumor cells was determine twice a week.

FuncS

Figure 6: Effect of NOTCH1 monoclonal antibody on colon tumor cells in vivo.

NOD/SCID mice injected with OMP-C9 colon tumor cells were treated with PBS or anti-NOTCH1 13M57 antibodies three days after cell injection, and the growth of tumor cells was determine twice a week.

FuncS

Figure 7: Effect of NOTCH1 monoclonal antibody 13M57 on luciferase-expressing PE-13 tumor cells.

Quantification of luciferase signal shows total tumor volume was significantly reduced (p=0.04) in animals treated with anti-NOTCH1 antibodies 13M57 compared to PBS and 5M108 injected controls.

FuncS

Figure 8: Effect of anti-NOTCH1 antibody 13M57 and chemotherapy combination therapy on breast tumor recurrence.

FuncS

Figure 9: Effect of combination therapy with anti-NOTCH1 antibody 13M57 and chemotherapy on colon tumor growth.

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