NeuroMab™ Anti-Plexin A1 Antibody,Clone NR3627P

Product Overview

Description

The antibody exhibits binding affinities only for mouse PlexinA1. This a novel anti-Plexin-A1 agonist antibody that promotes dendritic cell contraction.

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Rabbit

Clone Number

NR3627P

Applications

ELISA; In Vitro; Agonist

Relevant Diseases

Multiple sclerosis

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

PlexinA1

Official Name

PLXNA1

Full Name

Plexin-A1

Alternative Names

PLXNA1; NOV; NOVP; PLEXIN-A1; PLXN1; Plexin A1

Gene ID

5361(Human); 18844(Mouse); NCBI: NP_115618(Rat)

Uniprot ID

Q9UIW2(Human); P70206(Mouse)

Product Pictures

ELISA

Figure 1 is a graph showing the binding activity of an anti-mouse PlexinA1 antibody to mouse PlexinA1.

Sema3A(+) indicates the measured value when 0.8 μg/mL of mouse semaphorin 3A was added, and Sema3A(-) indicates the measured value when mouse semaphorin 3A was not added.

Antangonist

Figure 2 illustrates a graph showing the β-β measured by immobilizing the mouse chimeric antibodies hPANL#240-mFc, 359B-2-2-3-6-mFc, PXB693-mFc, PXB727-mFc, and PXB361b-mFc onto a Protein G biosensor. Plot of the binding reaction and applying human PlexinA1 and mouse PlexinA1 on it.

Sema3A(+) indicates the measured value when 0.8 μg/mL of mouse semaphorin 3A was added, and Sema3A(-) indicates the measured value when mouse semaphorin 3A was not added.

ELISA

Figure 3 illustrates a graph showing the dose-dependent semaphorin 3A-like agonistic activity of anti-PlexinA1 antibodies on contraction of mouse bone marrow-derived dendritic cells.

Sema3A(+) indicates the measured value when 0.8 μg/mL of mouse semaphorin 3A was added, and Sema3A(-) indicates the measured value when mouse semaphorin 3A was not added.

Figure 4 illustrates a graph showing the binding affinity of agonist antibody mouse chimeras PXB693 and PXB727 to mouse PlexinAl, mouse PleixinA2, and chimeric proteins of mouse PlexinAl and PlexinA2. 1: mouse PlexinA1 sema domain, 2: mouse PlexinA1/A2 sema domain chimera, 3: mouse PlexinA2 sema domain, Control Ab: control antibody, PXB693: mouse chimeric antibody PXB693-mFc, PBX727- mFc: mouse chimeric antibody PXB727.

Sema3A(+) indicates the measured value when 0.8 μg/mL of mouse semaphorin 3A was added, and Sema3A(-) indicates the measured value when mouse semaphorin 3A was not added.

Publications