Product Overview

Description

IL-8, also known as neutrophil chemotactic factor, neutrophil activating protein, and monocyte-derived neutrophil chemotactic factor, is a member of the alpha (C-X-C) subfamily of chemokines called CXCL8. In response to proinflammatory stimuli, IL-8 is produced by monocytes, macrophages, T cells, neutrophils, and fibroblasts. IL-8 promotes neutrophil chemotaxis and degranulation. The 72-amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. Recombinant human IL-8 is an 8,400 Da protein containing 72 amino acids. This antibody reacts with human Interleukin-8 (IL-8) protein. The immunogen used to produce the antibody is recombinant human IL-8 produced in E. coli containing 72 amino acids and having a molecular mass of 8,452 Da.

Immunogen

Purified recombinant human IL-8

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

Mouse IgG1

Clone Number

CBP2132

Applications

ELISA; WB

Application Notes

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several
factors, including secondary antibody affinity, antigen concentration,
sensitivity of detection method, temperature, and length of incubations,
etc. The suitability of this antibody for applications other than those
listed below has not been determined.
The following concentration ranges
are recommended starting points for this product.
ELISA: 0.1-1 µg/ml
Western blot: 1 µg/ml

Conjugation

Unconjugated

Product Properties

Concentration

1. 100 µg, 1 mg/ml in PBS, pH 7.4 with 0.02% sodium azide. 2. 500 µg, 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Purification

Protein G affinity column

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

IL-8

Official Name

CXCL8

Full Name

chemokine (C-X-C motif) ligand 8

Alternative Names

IL8/NAP1 form IV; GCP/IL-8 protein IV; NAF; T-cell chemotactic factor; 1-77; Ala-IL-8; Interleukin-8; IL-8; Neutrophil-activating protein 1; GCP/IL-8 protein II; IL8/NAP1 form II; GCP/IL-8 protein V; MDNCF; Protein 3-10C; Lymphocyte-derived neutrophil-activating factor; Neutrophil-activating factor; Granulocyte chemotactic protein 1; LYNAP; NAP-1; Monocyte-derived neutrophil chemotactic factor; 6-77; 7-77; C-X-C motif chemokine 8; GCP1; NAP1; Ser-IL-8; 5-77; GCP/IL-8 protein VI; IL8/NAP1 form I; IL8/NAP1 form VI; Monocyte-derived neutrophil-activating peptide; C-X-C motif; 8-77; 9-77; LUCT; Chemokine; GCP-1; MDNCF-b; MDNCF-c; IL8/NAP1 form V; LECT; IL8/NAP1 form III; GCP/IL-8 protein III; Emoctakin; GCP/IL-8 protein I; MONAP; IL8