Anti-CHIP Monoclonal Antibody (CBP3766)

[CAT#: NAB2012160LS]

Anti-CHIP Monoclonal Antibody

Host Species:: Rat

Species Reactivity:: Human; Mouse

Applications:: WB; IHC-P

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Product Overview

Description

CHIP is an E3 ubiquitin-protein ligase known to ubiquitinate misfolded chaperone-bound substrates and target them for proteasomal degradation. CHIP mediates polyubiquitination of DNA polymerase beta at 'Lys-41', 'Lys-61' and 'Lys-81', thereby plays a role in base-excision repair. CHIP can also negatively regulate the suppressive function of regulatory T-cells during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. CHIP also negatively regulates TGF-beta signaling by modulating the basal levels of SMAD3 via ubiquitin-mediated degradation. Mutations in CHIP cause spinocerebellar ataxia type 16.

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Rat

Isotype

IgG2b, κ

Clone Number

CBP3766

Applications

WB; IHC-P

Application Notes

WB (0.5-1.0 µg/mL); IHC-P (2.0-10 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CHIP

Official Name

CHIP

Full Name

Heat Shock Protein A Binding

Alternative Names

CHIP; STIP1 Homology And U-Box Containing Protein 1; Carboxy Terminus Of Hsp70-Interacting Protein; RING-Type E3 Ubiquitin Transferase CHIP; E3 Ubiquitin-Protein Ligase CHIP; CLL-Associated Antigen KW-8; Antigen NY-CO-7; Heat Shock Protein A Binding

Publications