VPAC2 Receptor Assay Cell Line
[CAT#: NCL2110P260]
- Species:
- Human
- Applications:
- GPCR Screening
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: VIPR2 cAMP Maxensor cell line
Official Full Name: Vasoactive intestinal peptide receptor 2
DNA Accession Number: NM_003382.4
Host Cell: U2OS
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen
Official Full Name: Vasoactive intestinal peptide receptor 2
DNA Accession Number: NM_003382.4
Host Cell: U2OS
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen
Features
The red cAMP Maxensor-VIPR2 contains U2OS cells stably expressing the red cAMP Maxensor biosensor and vasoactive intestinal peptide receptor 2 (unlabeled). Creative Biolabs' red cAMP Maxensor-VIPR2 cell line is designed to test compounds or analyze their ability to modulate vasoactive intestinal peptide receptor 2. When the agonist binds to VIPR2, the G protein is activated, which in turn triggers the camp. This cell line has been verified by measuring the increase of cAMP in the cytosol and analyzing the distribution of the red cAMP Maxensor biosensor in the cell. This cell line allows image analysis of compound-induced stimuli. In high-content analysis (HCA) and high-throughput analysis (HTA), the use of human PACAP-38 as an agonist validates this highly reproducible assay.
Cell Types
Other Cells
Applications
GPCR Screening
Application Notes
About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.
Research Areas
GPCR
Assay
VPAC2 Receptor Assay
Assay Description
The VPAC2 receptor detection (VIPR2) from Creative Biolabs uses Maxensor Biosensors technology to measure VIPR2 activation. VPAC2 receptor signals are stimulated by adenylate cyclase through the trimeric Gs protein, increased cAMP, and downstream L-type calcium channel interactions.
In this assay, we use the cAMP-Maxensor biosensor to measure receptor activation/inhibition through cAMP accumulation. The high reproducibility of this assay allows monitoring of the VPAC2 receptor activation process in living cells.
In this assay, we use the cAMP-Maxensor biosensor to measure receptor activation/inhibition through cAMP accumulation. The high reproducibility of this assay allows monitoring of the VPAC2 receptor activation process in living cells.
Assay Characteristics
Readout: cAMP Flux (Gs activation)
Agonist: PACAP-38
EC50 Agonist: 1.28 x 106-8 M for cAMP Flux
Type of Assay: Cell-Based/Functional
Detection Method: Fluorimetry
Agonist: PACAP-38
EC50 Agonist: 1.28 x 106-8 M for cAMP Flux
Type of Assay: Cell-Based/Functional
Detection Method: Fluorimetry
Species
Human
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
VIPR2
Full Name
Vpac2 Receptor
Alternative Names
VIPR2; C16DUPq36.3; DUP7q36.3; PACAP-R-3; PACAP-R3; VIP-R-2; VPAC2; VPAC2R; VPCAP2R; vasoactive intestinal peptide receptor 2
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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