VPAC2 Receptor Assay Cell Line

[CAT#: NCL2110P260]

Species:: Human

Applications:: GPCR Screening

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: VIPR2 cAMP Maxensor cell line
Official Full Name: Vasoactive intestinal peptide receptor 2
DNA Accession Number: NM_003382.4
Host Cell: U2OS
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen

Features

The red cAMP Maxensor-VIPR2 contains U2OS cells stably expressing the red cAMP Maxensor biosensor and vasoactive intestinal peptide receptor 2 (unlabeled). Creative Biolabs' red cAMP Maxensor-VIPR2 cell line is designed to test compounds or analyze their ability to modulate vasoactive intestinal peptide receptor 2. When the agonist binds to VIPR2, the G protein is activated, which in turn triggers the camp. This cell line has been verified by measuring the increase of cAMP in the cytosol and analyzing the distribution of the red cAMP Maxensor biosensor in the cell. This cell line allows image analysis of compound-induced stimuli. In high-content analysis (HCA) and high-throughput analysis (HTA), the use of human PACAP-38 as an agonist validates this highly reproducible assay.

Cell Types

Other Cells

Applications

GPCR Screening

Application Notes

About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.

Research Areas

GPCR

Assay

VPAC2 Receptor Assay

Assay Description

The VPAC2 receptor detection (VIPR2) from Creative Biolabs uses Maxensor Biosensors technology to measure VIPR2 activation. VPAC2 receptor signals are stimulated by adenylate cyclase through the trimeric Gs protein, increased cAMP, and downstream L-type calcium channel interactions.

In this assay, we use the cAMP-Maxensor biosensor to measure receptor activation/inhibition through cAMP accumulation. The high reproducibility of this assay allows monitoring of the VPAC2 receptor activation process in living cells.

Assay Characteristics

Readout: cAMP Flux (Gs activation)

Agonist: PACAP-38

EC50 Agonist: 1.28 x 10⁶-8 M for cAMP Flux

Type of Assay: Cell-Based/Functional

Detection Method: Fluorimetry

Species

Human

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.