Human Brain Medulloblastoma Cell (ONS-76)

Species:: Human

Applications:: Cell Culture

Cell Types:: Tumor Cells

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Product Overview

Features

Doubling time: 18.6 hours (CelloPub=CLPUB00094; PubMed=2504489; PubMed=2818910); ~24 hours (lot 08192008), ~32 hours (lot 12042014) (JCRB).

Microsatellite instability: Stable (MSS) (Sanger).

Cell Types

Tumor Cells

Applications

Cell Culture

Relevant Diseases

Medulloblastoma

Species

Human

Properties

Size

1x10^6

Form

Frozen

Culture Medium

DMEM high glucose medium + 10% FBS + 1% P/S

STR Analysis

Amelogenin X

CSF1PO 10,12

D2S1338 17,19

D3S1358 15

D5S818 9,10

D7S820 11,12

D8S1179 10

D13S317 8,13

D16S539 9,10

D18S51 14,21

D19S433 14,15.2

D21S11 29,30.3

FGA 24

Penta D 12,13

Penta E 15,16

TH01 9

TPOX 8

vWA 14,16

Biosafety Level

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Donor Information

Donor Health

Tumor

Publications

Publications (0)

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Scientific Resources

[Brochure]

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Have found them to be extremely reliable

The cells have shown consistent proliferation rates and viability across multiple experiments. Their genetic stability and responsiveness to treatments have enabled us to gather meaningful data. Moreover, the product was delivered in excellent condition, and customer service was very helpful.

Excellent

I would highly recommend this cell line to other researchers in the field

These cells are easy to handle and have a high proliferation rate, which is crucial for our high-throughput screening assays. The reproducibility of our results with these cells has been outstanding, and the technical support provided was top-notch.

Excellent

The ordering process was smooth, and the product arrived in perfect condition

ONS-76 cells have been a reliable model for our studies on medulloblastoma. They exhibit strong growth and viability, and their genetic profile is well-characterized. The cells have been responsive to various treatments, allowing us to gather reproducible and meaningful data.

Excellent

An excellent model for our cancer research

They are easy to culture and maintain, with high proliferation rates and consistent genetic expression. The reproducibility of our results with these cells has been outstanding, allowing us to draw meaningful conclusions from our experiments. The technical support and detailed documentation were very helpful.

Excellent

Customer support was also excellent, addressing our queries promptly

The ONS-76 cell line has been pivotal for our research on pediatric brain tumors. These cells have shown consistent growth patterns, and their genetic profile aligns well with published data. We were particularly impressed by their responsiveness to various experimental treatments.

Q&As

How often should I passage the ONS-76 cell line, and what is the recommended split ratio?

The ONS-76 cell line should be passaged when it reaches approximately 80% confluence, typically every 2-3 days. We recommend using a split ratio of 1:3 to 1:6, depending on your experimental needs. Regular passaging helps maintain healthy cells and ensures consistent experimental results.

Are there any known genetic mutations or characteristics specific to ONS-76 cells?

Yes, ONS-76 cells have been characterized for various genetic mutations and characteristics. These cells often exhibit alterations common to medulloblastomas, such as changes in the SHH (Sonic Hedgehog) signaling pathway, which is frequently implicated in the development of this tumor type. Detailed genetic profiles are available upon request, providing valuable information for researchers focusing on specific genetic aspects of medulloblastoma.

What are the key differences between ONS-76 cells and other medulloblastoma cell lines?

ONS-76 cells, like other medulloblastoma cell lines, have unique genetic and phenotypic profiles that reflect their tumor origin. Compared to other cell lines, ONS-76 may exhibit distinct growth characteristics, gene expression patterns, and responses to drugs. These differences make ONS-76 a valuable model for specific types of medulloblastoma research, particularly those related to the SHH pathway. Comparing multiple cell lines can provide a comprehensive understanding of the disease.