Cerebral Organoid Maturation Kit (Human)

[CAT#: NCC200610ZP] Review(5) Q&As(3)

Culture medium kit for extended maturation of human cerebral organoids

Applications:: Cell Culture; Cell Differentiation; Organoid Culture

Cell Types:: iPSC; Embryonic Stem Cell

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Product Overview

Description

The NeuroDiff™ Cerebral Organoid Kit (Cat. No. NCC200503ZP) is a defined, serum-free cell culture medium that can reliably produce brain organs derived from human pluripotent stem cells (hPSC) through a simple four-stage protocol. Cerebral organoids are three-dimensional in vitro models with in vitro composition, with cell composition and structural organization representing human brain development. Brain organoid formation begins through an intermediate embryoid body (EB) formation step, and then neuroepithelial cells are expanded. After maturity, organoids generated using this kit have cortical-like areas, such as ventricular area (PAX6 + / SOX2 + / Ki-67 +), extraventricular area (Ki-67 + / p-Vimentin +), middle area (TBR2 +) and cortical plate (CTIP2 + / MAP2 + / TBR1 + ), their layering directions are similar to those observed in vivo.

For longer organoid culture times (>40 days), the components required for organoid maturation can be purchased as the NeuroInduc™ Cerebral Organoid Maturation kit (Catalog#NCC200610ZP).

Cell Types

iPSC; Embryonic Stem Cell

Species Reactivity

Human

Applications

Cell Culture; Cell Differentiation; Organoid Culture

Research Areas

Stem Cell Research

Properties

Components

NeuroInduc™ Cerebral Organoid Basal Medium 2, 250 mL
NeuroInduc™ Cerebral Organoid Supplement E, 4.5 mL

Formulation

Serum-Free

Shelf Life

Stable until expiry date (EXP) on label.

Research Use Only

For research use only

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The inclusion of all necessary components saved us time and effort in sourcing individual reagents

The clear, step-by-step instructions made the protocol easy to follow, and we saw significant maturation in our cerebral organoids within the expected timeframe. The kit's reagents are high quality, and the results were consistent across multiple batches. This kit has significantly reduced the variability in our experiments, allowing for more reproducible data.

Excellent

Greatly enhanced the quality of our cerebral organoids

The protocol was user-friendly, and the detailed instructions ensured that even junior members of the lab could successfully execute the procedure. The matured organoids showed excellent viability and intricate neuronal networks, which are critical for our neurodevelopmental studies.

Excellent

This kit is a valuable tool

The reagents were easy to work with, and the maturation process was straightforward. The resulting organoids exhibited robust neural activity, which was confirmed by electrophysiological recordings.

Excellent

Our lab has seen a significant improvement in the quality of our cerebral organoids

The organoids matured quickly and displayed advanced neural differentiation, including the formation of synaptic connections. The kit's user-friendly protocol and high-quality reagents have made it a valuable asset to our research.

Excellent

Generated more accurate and reproducible data

Using the kit has significantly improved the maturation of our cerebral organoids. The protocol is well-designed, and the reagents are of high quality, resulting in organoids with complex neural architectures and functional synapses.

Q&As

How long does it take for organoids to mature using this kit?

The maturation process using the kit typically takes 4 to 8 weeks, depending on the specific protocol and desired level of maturity. This timeframe allows for the development of complex neural networks and advanced brain-like structures. The protocol provides guidance on optimizing conditions to achieve the best results in a reasonable timeframe.

What kind of neuronal and glial markers can I expect to express in the matured organoids?

Matured organoids typically express a range of neuronal markers such as MAP2, NeuN, and synaptophysin, as well as glial markers like GFAP and S100β. The kit promotes the development of diverse neural cell types, including cortical neurons and astrocytes, allowing for detailed studies of brain structure and function at both cellular and molecular levels.

Can I use this kit with any human pluripotent stem cell line?

Yes, the kit is compatible with most human pluripotent stem cell lines, including both embryonic stem cells and induced pluripotent stem cells. However, it is important to verify the quality and differentiation potential of your stem cell line, as variations can affect the efficiency of organoid maturation and the overall experimental outcome.