Mouse Anti-Cytokeratin 7/17 Monoclonal Antibody (CBP2293)

Description

Cytokeratins are proteins that are involved in the cytoskeleton. The cytokeratin proteins are heterotetramers composed of two type I and two type II subunits. Cytokeratin 17 is a type I protein and cytokeratin 7 is a type II intermediate filament protein, each protein contains three coiled-coil domains. Cytokeratin 7 has an approximate molecular weight of 54 kD; cytokeratin 17 has an approximate molecular weight of 46 kD. Cytokeratin 17 is expressed in the basal cells of complex epithelia, but not in stratified or simple epithelia. Cytokeratin 7 is expressed in the epidermis, bronchus, and mesothelium but not in the colon, exocervix or liver. Mutations in cytokeratin 17 have been shown to result in pachyonychia congenital. Cytokeratin 17 has been shown to interact with the EGF receptor. Cytokeratin 7 has been shown to interact with the EGF receptor and heat shock protein 70 kD1A. The C-46 monoclonal antibody recognizes human, bovine and porcine (but not mouse, rat, or rabbit) cytokeratin 7 and cytokeratin 17 and is useful for Western blotting.This antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.

Immunogen

Cytoskeleton preparation derived from Hela cells

Species Reactivity

Human; Bovine; Porcine

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2293

Applications

WB; IHC-P; IP; ICC

Application Notes

WB (1:500-1:2000)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Cytokeratin 7/17

Official Name

Cytokeratin 7/17

Full Name

Cytokeratin 7/17

Alternative Names

Keratin 7/keratin17; Keratin 7/17

1. Glass C, et al. 1988. J. Cell Biol. 107:1337.
2. Troyanovsky SM, et al. 1992. Eur. J. Cell Biol. 59:127.
3. Terrinoni A, et al. 2001. J. Invest. Dermatol. 117:1391.