Mouse Anti-Human E1 Ubiquitin Activating Enzyme Monoclonal Antibody (CBP2490)

[CAT#: NAB2010613LS]

Anti E1 Ubiquitin Activating Enzyme Monoclonal Antibody

Host Species:: Mouse

Species Reactivity:: Human

Applications:: WB; ICC

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Product Overview

Description

The proteasomal pathway of protein degradation involves two discrete steps: ubiquitination and degradation. Aside from proteasomal degradation, ubiquitination also plays an important role in transcriptional regulation, protein trafficking including endocytosis and lysosomal targeting, and the activation of kinases involved in signaling processes. Ubiquitination is carried out in three main steps: activation, conjugation, and ligation. E1 Ubiquitin Activating Enzyme (UBE1) initiates the cascade of conjugation by activating their respective modifiers. It is highly conserved in human, yeast, and plants. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair.

Immunogen

Mixture of human and mouse E1 protein.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2490

Applications

WB; ICC

Application Notes

WB (0.25-2.0 µg/mL); ICC (1.0 - 5.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C.

Handling Advice

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

E1 Enzyme

Official Name

E1 Ubiquitin Activating Enzyme

Full Name

E1 Ubiquitin Activating Enzyme

Alternative Names

E1 Ubiquitin Activating Enzyme; E1 enzymes

References

1. Xu W, et al. 2013. Curr. Pharm. Des. 18:3201.
2. Neutzner M, et al. 2012. Essays Biochem. 52:37.
3. Schulman BA, et al. 2009. Nat. Rev. Mol. Cell Biol. 10:319.
4. Salvat C, et al. 2000. Eur. J. Biochem. 267:3712.

Publications