Mouse Anti-Human β-NGF Monoclonal Antibody (CBP3718)

Description

β-NGF is a member of the neurotrophin family, which controls the growth, survival and maturation of neurons. In peripheral and central neurons, neurotrophin is an important regulator of nerve cell survival, differentiation and maintenance. They can be detected throughout the embryogenesis and adult stages of the central and peripheral nervous systems. In animal models, β-NGF can prevent neuronal degeneration and has become a therapeutic target in neuron regeneration research. β-NGF is also essential for the survival and maintenance of sympathetic and sensory neurons. It is the main neurotrophin that maintains the homeostasis of neurogenesis. It prevents neuronal apoptosis and promotes maturation while supporting axon branching. Two receptors are known to interact with NGF, p75NTR and TrkA. The NGF-TrkA signaling pathway induces two pathways by activating PI3K-Akt or Ras-MAPK. Either way can lead to CREB phosphorylation and increase the expression of anti-apoptotic genes to maintain the vitality of neurons. Animal models of neurodegenerative diseases show that NGF can promote the repair of myelin sheaths of neurons in the peripheral nervous system. Clinical studies have shown that abnormal NGF signaling is related to Alzheimer's disease.

Immunogen

Recombinant Human β-NGF

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a, κ

Clone Number

CBP3718

Applications

Neut

Application Notes

Neut (0.1-0.8 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Form

Liquid

Formulation

0.2 µm filtered in PBS, pH 7.2, containing no preservative.

Concentration

Batch Dependent

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

β-NGF

Official Name

NGFB

Full Name

Nerve growth factor

Alternative Names

NGF; Beta-HSAN5; NGFB; nerve growth factor

Gene ID

4803(Human); 18049(Mouse); 310738(Rat)

Uniprot ID

P01138(Human); P01139(Mouse); P25427(Rat)

1. Ullrich A, et al. 1983. Nature. 303:821.
2. Klein R, et al. 1991. Cell. 65:189.
3. Welker P, et al. 2000. Immunology 99:418.
4. Fahnestock M, et al. 2004. Prog. Brain Res. 146:101.
5. Bruno MA. Cuello AC. 2006. Proc. Natl. Acad. Sci. USA 103:6735.
6. Aloe L, et al. 2015. Curr. Neuropharmacol. 13:294.