Brain Ischemia/Hemorrhage/Stroke Modeling and Drug Efficacy Testing Service
Disease Mechanism
Cerebrovascular accidents are divided into two categories: hemorrhagic and ischemic. Brain hemorrhage is non-traumatic hemorrhage of the brain parenchyma (brain hemorrhage, subarachnoid hemorrhage). Brain ischemia is focal cerebral ischemia and infarction caused by insufficient blood supply or interruption of blood flow.
Modeling
| Classification | Modeling Method | Animals |
|---|---|---|
| Brain Ischemia/Hemorrhage/Stroke model | Reperfusion after ligation of both common carotid arteries for 5 minutes/reperfusion after ligation of internal carotid arteries and bilateral vertebral arteries (global cerebral ischemia) | Rat |
| MCAO suture method (focal cerebral ischemia) | Rat | |
| Ferric Chloride - Middle Artery Thrombosis | Rat | |
| Common carotid artery injection of complex thrombus-inducing agents (adenosine diphosphate, coagulant, and epinephrine) to cause platelet thrombus in capillaries and arterioles | Rat | |
| Autologous blood is drawn and injected into specific brain regions | Rat/Mouse | |
| Non-heparinized autologous blood-brain stereotaxic injection (multiple injections) | Rat/Mouse | |
| Heparin and collagenase mixed localized injection caused local oozing | Rat/Mouse | |
| Autologous blood injected into the cisterna magna mixed with cerebrospinal fluid (autologous blood, fresh arterial blood, and oxyhemoglobin) [subarachnoid hemorrhage] | Rat | |
| Coagulation knife injury or localized injection of paraffin oil (traumatic bleeding) | Rat/Mouse |
Assays after Modeling
Creative Biolabs offers a variety of well-accepted behavioral tests to assess potential anxiolytic, antidepressant, or antipsychotic effects, as well as cognitively enhancing and disruptive effects on learning and memory (cognitive, psychiatric, neurological). We have developed a comprehensive range of rat primary neuronal culture models and assays to evaluate the neurotoxic, neuroprotective or neurotrophic effects of test compounds (cellular models).
1) TTC staining is used to determine the volume of cerebral infarction
2) Longa scoring method, Garcia scoring method or Bederson scoring method, etc.
In additon to the above tests, we also provide MRI/PET-CT in vivo imaging of small animals, cardiac ultrasound, X-ray films, tissue fluorescence imaging (whole brain slide scan, tissue immunofluorescence imaging), patch clamp on cells and brain slices, multi-channel in vivo electrophysiological recordings, respiration recordings, behavioral testing of pain, anxiety, depression, olfactory function-related (go/no-go) in awake animals.
Why Creative Biolabs?
With extensive research experience, Creative Biolabs also helps our clients validate and develop the most cost-effective experimental designs and protocols. According to your target indications we can establish a screening program for your drug project. In-house expertise allows us to assist you in most CNS and PNS disorders. If you are interested in our service, please do not hesitate to contact us for more details.
