Cloned Channels and Receptors Recordings Assay

Cell Membrane Channels and Receptors

Cell surface receptors are special integral membrane proteins embedded on the surface of the cell membrane, which can receive signals from extracellular molecules, induce changes in cell metabolism and activity, and enable cell-to-cell communication. Cell surface receptors are divided into three categories according to their specific functions, ion channel-related receptors, enzyme-linked receptors and G protein-coupled receptors. These receptors work together to regulate almost all cell activities and determine cell fate.

Membrane channels are a family of proteins widely present on cell membranes, which provides energetically favorable channels for specific ions or other molecules to pass through lipid membranes with the characteristics of passive diffusion permeability, extremely high transmission rate, and selectivity. This ionic activity can generate transient dynamic potentials or maintain resting potentials, which greatly contributes to biofilm excitability and neural signaling processes.

Fig 1. Large-scale screening of cell membrane surface receptor interactions. (Jones, et al., 2018)

We Provide Cloned Channels and Receptors Recordings Assays

Channel and receptor proteins on the cell membranes control almost all cell behaviors and activities. Disordered and disturbed expression of these proteins often leads to a series of severe neurological or genetic diseases, including Parkinson's disease, Alzheimer's disease, even tumors. This makes the study of cell surface channels and receptors extremely important in pathology, pharmacy and biology.

Patch clamp and other electrobiological methods are the most commonly used methods for characterizing cell surface ion channels, and protein assay methods based on enzyme-linked reaction and immunological principles are often used for the detection of protein receptors and channel proteins. In addition, proteomics and gene editing are also commonly used research methods. We can complete the receptor recording assay for you through a range of means, covering every step you may need in drug development and biological research.

Fig 2. Characterization of calcium channels by calcium imaging. (Loser, et al., 2021)

Establish Appropriate Physiological Cell Models to Evaluate Drugs of Interest

Genetically engineered expression system cell lines are powerful tools for drug screening and biological research, with highlighted responses of specific ion channels, receptors, and signaling proteins. Primary cell culture models freshly isolated from native tissues can adequately reflect the functional roles of receptors and channels in their native environment. Well-characterized immortalized cell line culture models are easier to obtain, maintain, and more susceptible to molecular intervention. We provide the most suitable cell models for your experiments and evaluations, including

• Primary Cells
• Stable cell lines
• Transfected cells

Choosing Appropriate Electrophysiology Recording Methods

Various electrophysiological methods like sharp electrode, microelectrode array, etc. are used to screen the effects of various molecules on ion channels, of which the patch clamp technique is by far the most sensitive of all intracellular recording methods, providing various types of electrical activity information with high temporal resolution and signal-to-noise ratio. Patch-clamp technique establishes direct contact with the intracellular environment through penetration of glass micropipettes, and formates a high-resistance seal that minimizes perturbation of extracellular potential. Ruptured or perforated patch configuration record data faster and longer, and realize the characterization of various ion channels. Channel proteins we can characterize include, but are not limited to:

• Voltage-Gated Sodium Channel
• Voltage-Gated Calcium Channel
• Potassium Channel
• TRP Channel
• Ligand Gated Ion Channel
• Acid-sensing Ion Channel
• Cystic Fibrosis Transmembrane Conductance Regulator
• Hyperpolarization-activated, Cyclic Nucleotide-gated Channel

Fig 3. HEK cell current traces recorded by patch clamp technique. (Repedius, et al., 2022)

Apply Molecular Biology Methods if Needed

In various validation experiments, cultured cells often require further molecular biology interventions. We eliminate/enhance the function of specific channels or receptors and change their expression status through various transfection methods and gene editing methods. We also directly characterize and identify receptors or channels through various biological means.

Creative Biolabs currently provides professional membrane channel cloning and receptor recording assays from a variety of cell sources, contributing to your project for the deciphering of intercellular signaling, intracellular response pathways, or assisting your work for drug design and development, target determination and other applications. In addition to the above services, we also provide all kinds of equilibrium solutions and experimental reagents that can be used in experiments to customers all over the world.

If you need any services or want to learn more information, please do not hesitate to contact us.

References

1. Jones, E.M.; et al. A scalable, multiplexed assay for decoding receptor-ligand interactions. bioRxiv. 2018, 10: 358739.
2. Loser, D.; et al. Human neuronal signaling and communication assays to assess functional neurotoxicity. Archives of Toxicology. 2021, 95: 229-252.
3. Rapedius, M; et al. There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments. Frontiers in Molecular Neuroscience. 2022, 15: 982316.