Anti-Neurofilament Marker (CBP3938)

Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are the main component of neuron cytoskeleton, mainly used to provide structural support for axons and adjust the diameter of axons. There are three main NF subunits, and the names of these subunits are based on the apparent molecular weight of mammalian subunits on SDS-PAGE. The light or minimum NF (NF-L) operating speed is 68-70 kD. The operating frequency of medium or medium NF (NF-M) is about 145-160 kD, and the operating frequency of heavy or highest NF (NF-H) is about 200-220 kD. However, due to the highly charged C-terminal region of the molecule, the actual molecular weight of these proteins is significantly lower. The level of NF gene expression is related to the axon diameter, which controls the speed of electrical signals propagating along the axon. Mutant mice with NF abnormalities have a phenotype similar to amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful to distinguish between glial cells (positive for NF) and glial cells (negative for NF).

Immunogen

Homogenized hypothalami recovered from Fischer 344 rats.

Species Reactivity

Human; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgM, κ

Clone Number

CBP3938

Applications

IHC-P; WB; ICC; IHC-F

Application Notes

IHC (1.0- 5.0 µg/mL); WB (1.0- 5.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Neurofilament Marker

Official Name

NEFL; NEFM; NEFH

Full Name

Neurofilament Marker (pan axonal, cocktail)

Alternative Names

Syntaxin

Gene ID

4741(Human); 18040(Mouse); 24588(Rat)

1. Siedler D, et al. 2014. Front Cell Neurosci. 8:429.