Neurosphere Assay

Neurospheres are three-dimensional, free-floating cellular clusters derived from neural stem cells (NSC) or neural progenitor cells (NPC). These cellular structures serve as an invaluable model system for studying neurogenesis, and neuronal differentiation, and assessing the potential of NSPCs to give rise to various neural cell types.

Creative Biolabs is committed to offering you our specialized neurosphere assay service. We meticulously guide you through every step, from designing your experimental program to overseeing the result analysis, ensuring a comprehensive and reliable process to support your research endeavors.

Isolation and Culture of Neurosphere

To harness the potential of neurosphere assays, it is crucial to establish reliable neurosphere models. Creative Biolabs specializes in creating and optimizing neurosphere cultures. The process involves the following steps:

Characterization of Neurospheres

Characterization is a critical aspect of neurosphere assays, enabling researchers to assess the quality and potential of these cellular structures. Creative Biolabs offers a comprehensive range of services for neurosphere characterization:

• Morphological Analysis

The size and shape of neurospheres can provide insights into the state of the NSCs within. Smaller, uniform neurospheres often indicate a more homogeneous NSC population.

• Immunocytochemistry

Immunocytochemical analysis allows for the identification of specific neural cell markers within the neurospheres. This helps in confirming the presence of neural progenitors and differentiated neural cell types.

• Proliferation and Differentiation Potential

By subjecting neurospheres to appropriate differentiation conditions, researchers can evaluate the potential of NSCs to generate various neural cell types. This is a crucial step in understanding the versatility of the cell population.

Neurosphere Assays

Creative Biolabs provides a comprehensive array of meticulously designed neurosphere assay services for your research needs, each tailored to specific requirements:

• Differentiation Assays

By inducing differentiation within neurosphere cultures, researchers can study neural cell fate determination. Our differentiation assays are designed to assess the potential of neural stem cells to generate neurons, astrocytes, and oligodendrocytes.

• Toxicity Screening

Neurospheres serve as a reliable platform for assessing the neurotoxicity of chemicals, drugs, or environmental agents. Our neurosphere assays enable the evaluation of potential adverse effects on neural cells, aiding in safety assessment.

• Drug Efficacy Testing

The evaluation of pharmaceutical compounds on neurospheres provides critical insights into drug efficacy and potential therapeutic applications. Creative Biolabs offers tailored services for drug screening, helping advance drug discovery processes.

• Disease Modeling

Neurospheres can be derived from patient-specific iPSCs, allowing researchers to create disease models. These models are invaluable for investigating neurodegenerative diseases, such as Alzheimer's and Parkinson's, and exploring potential treatments.

Creative Biolabs offers a comprehensive suite of services, from establishing neurosphere models to conducting advanced neurosphere assays. Our commitment to excellence and innovation ensures that your neuroscience research is supported by the best-in-class resources and expertise.

Have not found what you need? Please do not hesitate to contact us directly, Creative Biolabs is committed to tailoring the most suitable service for your project's unique needs.