Neurosphere Assay
Neurospheres are three-dimensional, free-floating cellular clusters derived from neural stem cells (NSC) or neural progenitor cells (NPC). These cellular structures serve as an invaluable model system for studying neurogenesis, and neuronal differentiation, and assessing the potential of NSPCs to give rise to various neural cell types.
Creative Biolabs is committed to offering you our specialized neurosphere assay service. We meticulously guide you through every step, from designing your experimental program to overseeing the result analysis, ensuring a comprehensive and reliable process to support your research endeavors.
Isolation and Culture of Neurosphere
To harness the potential of neurosphere assays, it is crucial to establish reliable neurosphere models. Creative Biolabs specializes in creating and optimizing neurosphere cultures. The process involves the following steps:
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| Isolation of NSCs | Proliferation and Culture of NSCs | Differentiation and Transplantation | High-throughput Neurosphere System |
| NSCs can be obtained from mouse tissue at various stages of neurodevelopment using an innovative, optimized technique. Additionally, human embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) can be induced to differentiate into NSCs and then cultivated to generate neurospheres. | NSCs sourced from various origins are cultivated in a specialized proliferation medium enriched with EGF and FGF-2, tailored for the growth of neural stem cells. Depending on the specific requirements of the experiment, NSCs can be expanded in either 3D neurospheres or as 2D monolayer cultures. | To facilitate the differentiation of NSCs, EGF, and FGF-2 are frequently added to promote the maturation of distinct types of neurons and glial cells. Naive or differentiated neurospheres that have undergone proliferation can be transplanted into the CNS of experimental animals to assess their functionality in disease models. | The High-throughput Neurosphere System produces brain region-specific neurospheres for high-throughput assays, comprised of various combinations of neuronal and glial cells. |
Characterization of Neurospheres
Characterization is a critical aspect of neurosphere assays, enabling researchers to assess the quality and potential of these cellular structures. Creative Biolabs offers a comprehensive range of services for neurosphere characterization:
- Morphological Analysis
The size and shape of neurospheres can provide insights into the state of the NSCs within. Smaller, uniform neurospheres often indicate a more homogeneous NSC population.
- Immunocytochemistry
Immunocytochemical analysis allows for the identification of specific neural cell markers within the neurospheres. This helps in confirming the presence of neural progenitors and differentiated neural cell types.
- Proliferation and Differentiation Potential
By subjecting neurospheres to appropriate differentiation conditions, researchers can evaluate the potential of NSCs to generate various neural cell types. This is a crucial step in understanding the versatility of the cell population.
Neurosphere Assays
Creative Biolabs provides a comprehensive array of meticulously designed neurosphere assay services for your research needs, each tailored to specific requirements:
- Differentiation Assays
By inducing differentiation within neurosphere cultures, researchers can study neural cell fate determination. Our differentiation assays are designed to assess the potential of neural stem cells to generate neurons, astrocytes, and oligodendrocytes.
- Toxicity Screening
Neurospheres serve as a reliable platform for assessing the neurotoxicity of chemicals, drugs, or environmental agents. Our neurosphere assays enable the evaluation of potential adverse effects on neural cells, aiding in safety assessment.
- Drug Efficacy Testing
The evaluation of pharmaceutical compounds on neurospheres provides critical insights into drug efficacy and potential therapeutic applications. Creative Biolabs offers tailored services for drug screening, helping advance drug discovery processes.
- Disease Modeling
Neurospheres can be derived from patient-specific iPSCs, allowing researchers to create disease models. These models are invaluable for investigating neurodegenerative diseases, such as Alzheimer's and Parkinson's, and exploring potential treatments.
Creative Biolabs offers a comprehensive suite of services, from establishing neurosphere models to conducting advanced neurosphere assays. Our commitment to excellence and innovation ensures that your neuroscience research is supported by the best-in-class resources and expertise.
Have not found what you need? Please do not hesitate to contact us directly, Creative Biolabs is committed to tailoring the most suitable service for your project's unique needs.
