3D Neural Spheroid Model Development Service

Disease and injury in the central nervous system (CNS) have tremendous impacts on quality of life, and there is a high demand for models to study CNS pathologies and therapeutic strategies. Neurons and glial cells in the brain form a complex three-dimensional (3D), intertwined system and, therefore, engineering the microenvironment for in vitro investigations presents many inherent challenges. Microenvironmental cues, such as cellular, biochemical, mechanical, and topographical cues, can influence cell behaviors, including viability, proliferation, differentiation, migration, and protein and gene expressions. Notably, many native microenvironmental cues are lost in traditional two-dimensional (2D) cultures, which has led to the development of 3D in vitro models of the nervous system.

3D in vitro neural models provided by Creative Biolabs can bridge the gap between traditional 2D cultures and in vivo models.

Why 3D Neural Spheroid Models?

The nervous system functions by signal transmission between different circuits. Signal transduction relies on highly complex 3D networks. Understanding signal transduction through 3D neural spheroid disease models may provide deep insights into the pathophysiology of neurological disorders. In addition, the spheroids recapitulate several key features of the in vivo neural tissue, including cell types, cell morphology, circuitry formation, extracellular matrix (ECM) production, and mechanical properties. This 3D culture can serve as an attractive tool to study neurological regeneration, screen therapeutic agents, and gain insights into the organization, function, and development of the CNS.

Fig.1 Applications of 3D neural spheroids.

3D Neural Spheroid Model Development Service

At Creative Biolabs, we have a state-of-the-art cell culture platform and specialized neurobiological science technology. Neural cells cultured by our technicians through self-assembly into spheroid structures, which contain neurons, glial cells, and cell-synthesized matrices that are mechanically similar to the in vivo microenvironment and are electrically active. Our outstanding neural spheroid 3D model development service consists of the following steps (Fig.2).

Fig.2 3D Neural Spheroid immunofluorescence staining, neurons (red), astrocytes (green), and neural progenitor cells (cyan).

Creative Biolabs is honored to collaborate with neuroscience researchers around the world to provide relatively large numbers of 3D microtissues including 3D neural organoids for you to study CNS and neurological disorders. Is this what you are looking for? Please contact us directly for more information and technical support.