PETC Model Assay related Service
Creative Biolabs recently developed conditionally immortalized renal proximal tubular epithelial cells (iN-PTEC), a human kidney cell line that mimics kidney proximal tubular cells. These cells participate in the efficient transport of drugs and toxic compounds. iN-PTEC exhibits functional influx and efflux drug transporters (OAT1, OCT2, P-gp, MRP4, BCRP) and possesses proximal tubular characteristics.
This is the original version of iN-PTEC. It is well-characterized through stable transfection with SV40T and hTERT genes, and maintenance of megalin/cubilin-mediated endocytic and sodium-phosphate reabsorption. Additionally, it expresses DDP 4, ZO-1, and ALP as markers for proximal tubules, as well as CD338, MRP2, MRP4, OCT2, P-GP, and several enzymes.
Based on iN-PTEC 1.0, this version also uptake through organic anion transporter 1 (SLC22A6).
Based on iN-PTEC 1.0, this version also uptake through organic anion transporter 3 (SLC22A8).
iN-PTEC Based Assays
Based on the iN-PTEC models, our experts successfully developed an array of cell-based assays with minimal inter-laboratory variation to meet your objectives, which including but not limited to:
- Cytokine Production Analysis
- 3D Microfluidic Culture Analysis
- Ciliary Expression Analysis
- Gene Expression Analysis
- LDH Release Analysis
- NAG Release Analysis
- miRNA Secretion Analysis
- ATP Production Analysis
- Lactate Production Analysis
- O2 Consumption Analysis
- Genetic Modification Analysis
- Transporter Activity Analysis
- Receptor-Mediated Endocytosis (Megalin/Cubilin) Analysis
- Metabolic Enzyme Function & Expression (CYP, UGT, GST) Analysis
- ROS Production Analysis
iN-PTEC expresses the most essential renal drug transporters and is a valuable tool for predicting the pharmacokinetics, drug interactions, and nephrotoxicity of prospective therapeutic molecules.
- Regulatory Guidelines for Drug Elimination Routes
Recently, the FDA and the EMA have issued guidelines for the pharmaceutical industry. These guidelines suggest that drug development should include the characterization of drug-drug interactions (DDIs) and the identification of elimination pathways through drug transport proteins. This includes studies on renal drug transport at the sites of organic anion transporters 1 and 3 (OAT1, OAT3), multidrug and toxin extrusion transport 1 and 2 (MATE1, MATE2-k), p-glycoprotein (P-GP), breast cancer resistance protein (BCRP), and organic cation transporter 2 (OCT2). Our iN-PTEC systems encompass all these transporters.
The iN-PTEC system, with its proximal renal tubular epithelial features, complete drug transport function, expression of biomarkers following hazardous exposure, complete endocytic transport, and expression of renal drug metabolizing enzymes, is indeed well-suited for drug interaction research. It is compatible with high-throughput screening, which can generate reliable data. For more detailed information or specific inquiries, please feel free to contact us to discuss directly with our experts. We are here to assist you.
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