Drug-induced Renal Toxicity Assay related Service

Creative Biolabs provides valuable lead optimization services to help drug candidates move through the clinical development process while avoiding costly failures. Drug-induced nephrotoxicity is one such safety assessment that can be evaluated early in drug discovery to determine the potential of a compound to cause kidney-specific injury. We have developed a series of iN-PTEC based cell assays, non-cell-based assays, and biomarkers assays.

Cell-based Assays

• Cell viability and toxicity: Choosing a cell viability or cytotoxicity assay among the numerous options available can be a challenging task. We provide a variety of related assays, including but not limited to real-time cell viability, MTT/WST, LDH, and ATP assays.
• Oxidative stress: Under oxidative stress, the formation of reactive oxygen species (ROS) surges, causing changes in membrane lipids, proteins, and nucleic acids. We offer oxidative stress assays, including generalized oxidative stress assay, lipid peroxidation assay, selective ROS oxidative stress assay, glutathione assay, and so on.
• Multiparametric assay: Collaborate with us to offer multiparametric cell-based assays for your research, including but not limited to: cytoskeletal organization, mitochondria, and nuclear morphology.
• Drug transporters assay: Our newly developed iN-PTEC express the most relevant renal drug transporters (OAT1, OAT3, OCT2, PGP, MRP4, and BCRP). It is a useful tool for estimating the pharmacokinetics, drug interactions, and nephrotoxicity of potential therapeutic compounds.
• Internalization of a drug: The drug's mechanism of action includes inducing the internalization and turnover of cell surface receptors, which leads to receptor downregulation and endocytosis. Receptor-mediated endocytosis is an endocytic mechanism in which specific molecules enter cells via highly selective receptor-ligand interactions. This receptor-mediated internalization has significant potential for a variety of therapeutic and diagnostic applications.

Non-cell-based Assays

• Renal vesicular transport assay: Our vesicles have a greater dynamic range than other vesicles on the market. Use our vesicle transport assays to conduct medication interaction, inhibition, substrate, and pharmacokinetic research.

Biomarkers assays

• NAG assay: N-acetyl-glucoseaminidase (NAG) is the most often used urine enzyme assay for evaluating renal impairment and detecting nephrotoxicity. Our NAG assay employs fluorescence, colorimetric, spectrophotometric, and test strip methodologies.
• HO-1 expression: Heme oxygenase 1 (HO-1) is induced by a variety of stimuli. These include oxidative stress conditions, inflammatory factors, TGF-beta, and heat shock. Our HO-1 expression assay generates highly sensitive, repeatable, and accurate findings.

To bring more effective drugs to market, we assist researchers in reliably identifying and eliminating nephrotoxic chemicals during the preclinical stages of drug development. Contact us to discuss your requirements with one of our experts.