3D Retinal Organoid Model Development Service
At Creative Biolabs, we have successfully created a retinal platform that offers retinal organoids and retinal pigment epithelium (RPE) derived from iPSCs obtained from the same donor. Our models are developed using ethically sourced samples from both healthy donors and patients. This innovative platform provides researchers with a powerful tool for studying various aspects of retinal biology and advancing the understanding of neurological diseases.
Neurological Disease-based Retinal Organoid Modeling
Neurological diseases affecting the retina, such as retinitis pigmentosa and age-related macular degeneration. Our advanced retinal organoid models can be specifically designed to recapitulate the pathogenesis of these diseases. By utilizing iPSC technology, Creative Biolabs has the capability to generate iPSC-derived retinal organoids and RPE from samples provided by patients. This allows for the development of personalized models that accurately represent the diseases being studied.
Specifically, we can generate the patient-specific models for the diseases list below.
- Age-related macular degeneration (AMD): Wet AMD, Dry AMD
- Inherited Retinal Diseases (IRD)
- Retinitis pigmentosa (RP)
- Retinal vein occlusion (RVO)
- Leber congenital amaurosis (LCA)
- Stargardt disease,
- Usher's syndrome
Gene Editing for iPSC-derived Retinal Organoid
At Creative Biolabs, we leverage powerful gene editing techniques, such as CRISPR/Cas9 to introduce precise genetic modifications into iPSCs derived from patients with retinal diseases. By targeting disease-causing mutations or genetic risk factors, we can generate iPSC lines with specific genetic alterations, which are then differentiated into retinal organoids.
Our iPSC-derived organoids have been used to model the effect of the following dominant mutations in retinal diseases.
| Retinal Diseases | Targets |
| Leber congenital amaurosis (LCA) | RPE65 |
| CEP290 | |
| Retinitis pigmentosa (RP) | RPGR |
| MERTK | |
| PRPF31 | |
| Leber's hereditary optic neuropathy (LHON) | MT-ND4 |
| Age-related macular degeneration (AMD) | CFH |
| VEGF | |
| Usher syndrome | USH2A |
Retinal Organoid Characterization
Accurate characterization of retinal organoids is crucial for their utility in research and drug discovery. Our comprehensive characterization service encompasses a wide range of assessments to ensure the fidelity and functionality of the model system.
Highlights
Morphological Analysis
We employ advanced imaging techniques, such as confocal microscopy and electron microscopy, to visualize the cellular organization and structural integrity of retinal organoids. Detailed morphological analysis provides valuable insights into the formation of retinal layers, including the photoreceptor layer, inner nuclear layer, and ganglion cell layer.
Immunohistochemistry
Immunohistochemical staining allows us to examine the expression patterns of specific retinal cell markers within the organoid structure. By labeling key cell types, such as photoreceptors, bipolar cells, and Müller glial cells, we can assess the differentiation efficiency and the presence of disease-related phenotypes in the retinal organoids.
Functional Assays
We employ a range of functional assays, including electrophysiology, calcium imaging, cytotoxicity assays, cytokine release to measure the responsiveness of retinal cells to light stimuli and neuronal activity. These assays enable the investigation of disease-related functional impairments and the evaluation of drug candidates.
Our 3D Retinal Organoid Model Development Service offers several key advantages:
Patient-specific Modeling
By utilizing patient-derived iPSCs, we can create personalized retinal organoid models that faithfully recapitulate the genetic and cellular characteristics of individual patients.
Comprehensive Characterization
Techniques allow for in-depth analysis of retinal organoid structure and function.
Versatile Applications
Our retinal organoid models can be used for a wide range of applications, including basic research, drug screening, toxicity testing, and precision medicine.
Options for Using Our Retinal Organoid Model
Creative Biolabs provides flexible options for utilizing our retinal organoid models:
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Order organoids for use in your lab
If you are interested in ordering organoids for your lab, we offer a convenient and flexible supply of retinal organoids. We have regular batch releases every 4-6 weeks. In addition to fresh organoids, we also provide retinal organoids as cell pellets, RNA, and frozen sections slides, catering to various applications in the lab.
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Outsource your experiments to us
We offer a swift and dependable service that involves collaborative experiment design to address your specific inquiries, delivering results within a span of 2 months. Our tailored projects are conducted within our cutting-edge facilities.
For further information or to initiate a discussion regarding your unique requirements, please reach out to our team of specialists. They are well-versed in the field and are ready to assist you with any questions you may have. Contact us today to explore the possibilities of collaborating with our organization.
