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Human Brain Slices Recording Assay

Electrophysiological recordings of brain slices are a valuable technique for studying synaptic plasticity, which boost neuronal circuitry studies through functional brain tissue imaging. Up- or down-regulation of synaptic transmission can reveal cellular and network mechanisms in neurodegenerative diseases such as Alzheimer's disease and amyotrophic lateral sclerosis (ALS).

Creative Biolabs is the world-leading CRO for in vitro electrophysiological assays dedicated to neurodegenerative diseases drug discovery.

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We are able to perform electrophysiological recordings on human acute brain slices from two brain regions:

- Hippocampus

- Cortex (various areas)

- Striatum

- Thalamus

- Cerebellum

- Amygdala

- Substantia Nigra

We can perform electrophysiological recordings from:

- Pyramidal neurons

- Dentate gyrus granule neurons

- Interneurons.

Our Brain Slice Models

- Acute brain slices

- Organotypic brain slices

Neurological Disease-Specific Samples

- Disease Brain Slices Models: Our human brain tissues target specific neurological diseases, such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis (ALS), multiple sclerosis, and others. By analyzing brain tissue samples from individuals diagnosed with these diseases, researchers can identify biomarkers, study disease progression, and elucidate the molecular pathways involved.

- Aged Brain Slices Models: Brain slices from older animals are used to investigate age-related changes in neural circuits, synaptic plasticity, and neuronal excitability.

- Transgenic Brain Slices Modes: Brain slices from transgenic animals expressing specific genes or mutations related to specific neurological diseases can provide insights into the role of those genes in brain function and disorders.

General Workflow for Brain Slice Electrophysiological Recordings

Step 1 Brain Slice Preparation

Fresh brain tissue is obtained, usually from animals like rodents. The brain is sliced into thin sections, typically around 200 to 500 micrometers thick, using a specialized instrument called a vibratome. These slices retain the neural architecture and allow researchers to access specific brain regions.

Step 2 Recording Setup

Transfer a brain slice to a recording chamber that is continuously perfused with oxygenated ACSF.
Secure the slice using a nylon mesh and fine tungsten wires, allowing the tissue to stay submerged while being stabilized.

Step 3 Electrophysiological Recordings

Begin recording electrical signals (e.g., action potentials, postsynaptic potentials) from the neurons.
Adjust amplifier settings and filters to optimize signal quality.

Step 4 Data Collection and Analysis

Record data on synaptic responses, neuronal activity, or other relevant measurements during and after interventions.
Analyze collected data using appropriate software and statistical methods to derive meaningful insights.

Sample Data

For Research Use Only. Not For Clinical Use.