Human VIM Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP279]

Human VIM knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human VIM has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Research Areas

Neurogenesis

Species

Human

Properties

Form

Vimentin is found in connective tissue and in the cytoskeleton.

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8, 12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

Vimentin

Official Name

VIM

Alternative Names

VIM; CTRCT30; HEL113; vimentin

Gene ID

7431(Human); 22352(Mouse); 81818(Rat)

Uniprot ID

P08670(Human); P20152(Mouse); P31000(Rat)

Publications

Publications (0)

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