iNeu™ Human Hippocampal Spheroid

An Innovative Tool for Stratifying Alzheimer Disease Patient-Specific Cellular Phenotypes and Developing Therapies

Species:: Human

Applications:: High Throughput Screening

Cell Types:: Hippocampal Neuron; Spheroid

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Product Overview

Description

The hippocampus is important for memory formation and is severely affected in the brain of Alzheimer's disease (AD). When used in AD models, both APP and the atypical PS1 variant HS showed increased Aβ42/Aβ40 peptide ratio and decreased synaptic protein levels, which are common features of AD. However, these two variants differ in terms of tau hyperphosphorylation, protein aggregation and protein network changes. NeuroD1-mediated gene therapy in HSD-derived progenitor cells can regulate the expression of many genes, including genes related to synaptic transmission. Therefore, HS can be used to reveal the underlying mechanism of early pathogenic changes in the hippocampus of AD patients and provide a powerful platform for the development of treatment strategies for early AD.

Creative Biolabs's Human iPSC-Derived Hippocampal Spheroids are derived from induced pluripotent stem cells (iPSC) without integration under fully defined neural induction conditions.

Creative Biolabs's Human iPSC-Derived Hippocampal Spheroids are rich in hippocampal neurons and express zinc finger and BTB domain-containing protein 20 (ZBTB20) and Prospero homeobox protein 1 (PROX1). In addition, the hippocampal neurons derived from the APP variant HS exhibit profound transcriptome changes, which can be regulated by the overexpression of NeuroD1 (ND1), leading to the upregulation of genes whose gene products are related to synaptic transmission and are altered in AD.

Cell Types

Hippocampal Neuron; Spheroid

Applications

High Throughput Screening

Application Notes

1.hiPSC-derived HSs can be utilized as a source of hippocampal neurons
2.hiPSC-derived HSs can be used to model Alzheimer disease
3.hiPSC-derived HSs can be used to develop innovative therapeutic solutions

Relevant Diseases

Alzheimer's Disease

Species

Human

Properties

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.

References

Pomeshchik, Y., Klementieva, O., Gil, J., Martinsson, I., Hansen, M. G., de Vries, T., ... & Roybon, L. (2020). Human iPSC-Derived Hippocampal Spheroids: An Innovative Tool for Stratifying Alzheimer Disease Patient-Specific Cellular Phenotypes and Developing Therapies. Stem cell reports, 15(1), 256-273.

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The spheroids are easy to work with, and the results are both reproducible and reliable

The consistency across batches is remarkable, allowing us to obtain reliable and reproducible data. These spheroids closely mimic the in vivo environment, making them an invaluable tool for our drug discovery projects.

Excellent

The data quality is excellent

They maintained their 3D architecture throughout our long-term culture, which is crucial for our neurotoxicity studies. The results we obtained were consistent and aligned with our in vivo models.

Excellent

We’ve seen a significant improvement

These spheroids have significantly improved the efficiency of our high throughput screening. The human hippocampal spheroids provided a robust model for assessing neuroprotective compounds, allowing us to screen a large number of candidates in a relatively short time.

Excellent

The reproducibility between experiments is excellent

The human hippocampal spheroids are a fantastic model for studying neurodegenerative diseases. We used them to assess the effects of various compounds on synaptic health, and the data we collected was highly reliable. The spheroids responded well to treatments, making our experiments much more predictive.

Excellent

A significant advantage over traditional 2D cultures

They replicate the complexity of human brain tissue very well, providing a more physiologically relevant model compared to traditional 2D cultures.

Q&As

Are there any specific assays or experimental techniques that work particularly well with these spheroids?

Human hippocampal spheroids are well-suited for various assays, including neurotoxicity testing, drug screening, and neuroinflammation studies. Techniques such as immunohistochemistry, live-cell imaging, and high-content screening can be effectively used with these spheroids to assess cellular responses and interactions in a more physiologically relevant context.

What types of compounds or treatments can be tested using the human hippocampal spheroids?

The spheroids are versatile and can be used to test a wide range of compounds, including potential neuroprotective agents, neurotoxic substances, and drug candidates. They are particularly useful for studying the effects of compounds on synaptic health, neuronal survival, and other aspects of brain function and pathology.

How do I prepare the spheroids for use in high throughput screening assays?

Prepare the spheroids by gently transferring them from their culture vessels to the assay plates using appropriate tools to avoid damaging their structure. Ensure that the assay conditions, including media composition and incubation parameters, are optimized for the spheroids. Follow the recommended protocols for assay setup to achieve reliable and consistent results.