LPA3 Lysophospholipid Receptor Assay Cell Line
[CAT#: NCL2110P285]
- Cell Types:
- Other Cells
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: LPA3 (EDG7)/U2OS
Official Full Name: Lysophosphatidic acid receptor 3
DNA Accession Number: GenBank: AY322547
Host Cell: U2OS
Format: 2 cryopreserved vials
Resistance: G418
Storage: Liquid Nitrogen
Official Full Name: Lysophosphatidic acid receptor 3
DNA Accession Number: GenBank: AY322547
Host Cell: U2OS
Format: 2 cryopreserved vials
Resistance: G418
Storage: Liquid Nitrogen
Features
The HiTector LPA3 contains U2OS cells stably expressing unlabeled human lysophosphatidic acid receptor 3 (LPA3). Creative Biolabs' HiTector LPA3 cell line is designed to test compounds or analyze their ability to modulate lysophosphatidic acid receptor 3. When the agonist binds to LPA3, the G protein is activated, which triggers a cellular response mediated by the second messenger (calcium). This cell line has been verified by measuring the increase in calcium in the cytosol. The high reproducibility of this assay allows monitoring of the LPA3 activation process in high-throughput screening.
Cell Types
Other Cells
Research Areas
GPCR
Assay
LPA3 Lysophospholipid Receptor Assay
Assay Description
The LPA3 Lysophospholipid Receptor Assay from Creative Biolabs allows compounds to be tested or analyzed for their ability to modulate LPAR3. When the ligand binds to LPAR3, it activates the G protein, which in turn triggers a cellular response that contains calcium. Our LPAR3 test uses LPA as a positive control to measure changes in cytoplasmic calcium concentration. The high reproducibility of this assay allows monitoring of the LPAR3 activation/inhibition process in high-throughput screening.
Assay Characteristics
Readout: Calcium Flux
Agonist: LPA
EC50 Agonist: 1.93 x 106-6 M for Calcium Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Agonist: LPA
EC50 Agonist: 1.93 x 106-6 M for Calcium Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
LPAR3
Full Name
Lpa3 Lysophospholipid Receptor
Alternative Names
LPAR3; EDG7; Edg-7; GPCR; HOFNH30; LP-A3; LPA3; RP4-678I3; lysophosphatidic acid receptor 3
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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