- Host Species:
- Mouse
- Species Reactivity:
- Human
- Applications:
- DB; ELISA; FC; IHC; In Vitro
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Lot Number
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Lot Number
SPECIFIC INQUIRY
inquiryProduct Overview
Description
The antibody has a high affinity for aggregated forms of α-synuclein and a low affinity for monomeric forms of α-synuclein. The antibody is useful in the diagnosis of neurodegenerative diseases.
Species Reactivity
Human
Clonality
Monoclonal
Host Species
Mouse
Applications
DB; ELISA; FC; IHC; In Vitro
Relevant Diseases
Parkinson's Disease
Product Properties
Formulation
PBS only
Preservatives
BSA Free
Concentration
1mg/mL
Purification
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
Purity
>95% by SDS PAGE or HPLC
Endotoxin Level
Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg
Low Endotoxin < 1 EU/mg
Shipping
Gel Packs
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Research Use Only
For research use only
Target
Product Pictures
ELISA
Figure 1 shows the results of inhibition ELISA using this antibody.
Rats were treated with intraocular injections of 3H4 Fab solution (2.4 pg/eye) compared to sham or vehicle controls. Statistical significance is shown.
DB
Figure 2 shows the Pepscan results of a dot blot epitope assay for the monoclonal antibodies Syn-F1, Syn-F2, Syn-O1, Syn-O2, Syn-O3 and Syn-O4, and the Syn-1 antibody as a control.
Rats were treated with intraocular injections of 3H4 Fab solution (2.4 pg/eye) compared to sham or vehicle controls. Statistical significance is shown.
IHC
Figure 3 shows immunohistochemical analysis of Parkinson's disease tissue.
Rats were treated with intraocular injections of 3H4 Fab solution (2.4 pg/eye) compared to sham or vehicle controls. Statistical significance is shown.
FuncS
Figure 4 shows the percentage of dot-positive cells for each antibody tested.
Rats were treated with intraocular injections of 3H4 Fab solution (2.4 pg/eye) compared to sham or vehicle controls. Statistical significance is shown.
Publications
Publications (0)
Neural Antibodies
Scientific Resources
Customer Reviews and Q&As
Customer Reviews
Average Customer Ratings
Overall
5.0
ExcellentThe antibody's specificity and sensitivity are outstanding, providing clear and distinct stainingIt has significantly enhanced our ability to detect alpha-synuclein aggregation in brain tissues, leading to more reliable results.
ExcellentIts high affinity for alpha-synuclein ensures reproducible and consistent resultsThe antibody provides excellent specificity and minimal background noise, allowing us to obtain clear and precise data. It has greatly facilitated our research on the cellular mechanisms underlying Parkinson's disease.
ExcellentAn invaluable tool for our researchThe antibody produced sharp and clear images, allowing us to visualize alpha-synuclein distribution accurately. Its performance has been consistently reliable.
ExcellentIts consistent performance and reliability have made it a favorite in our labIt offers high sensitivity and specificity. We have consistently obtained accurate and reproducible results.
ExcellentIt has been a reliable and effective tool in our researchUsing the antibody for flow cytometry has greatly enhanced our ability to analyze alpha-synuclein expression in various cell types. The antibody's specificity and low background staining make it ideal for our studies.
Q&As
How does the specificity of Clone NR3855P?
Clone NR3855P exhibits exceptional specificity for alpha-synuclein, distinguishing it from other proteins with high accuracy. This antibody has been rigorously tested to ensure minimal cross-reactivity with other synuclein family members, such as beta-synuclein and gamma-synuclein. Researchers can rely on Clone NR3855P for precise detection of alpha-synuclein in complex biological samples, reducing the likelihood of false positives and enhancing the validity of their findings.
What are the storage conditions for the antibody to ensure its stability and longevity?
To ensure the stability and longevity of the antibody, it should be stored at -20°C upon receipt. For short-term storage (up to one month), it can be kept at 4°C. Avoid repeated freeze-thaw cycles as they can degrade the antibody's performance. Proper storage conditions will maintain the antibody’s efficacy and reliability for long-term use.
Are there any recommended protocols for using Clone NR3855P in immunohistochemistry?
Yes, we provide detailed protocols for using Clone NR3855P in immunohistochemistry. For optimal results, we recommend using a 1:200 to 1:500 dilution range, depending on the tissue type and antigen retrieval method. Standard protocols include antigen retrieval using citrate buffer (pH 6.0) and incubation with the antibody at 4°C overnight. These guidelines help achieve robust staining and reproducible results.
For Research Use Only. Not For Clinical Use.
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