NeuroMab™ Anti-Alpha Synuclein Antibody(NRP-0422-P632)

Product Overview

Description

NRP-0422-P632(IgG2, immunogen: amino acids 118-126 of α-synuclein, n-linker). The antibody is useful, for example, for treating and/or diagnosing disorders associated with α-synuclein, particularly accumulation of α-synuclein deposits.

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Applications

ELISA; WB; IP; In Vitro

Relevant Diseases

Parkinson's Disease

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

AlphaSynuclein

Official Name

SNCA

Full Name

Alpha-synuclein

Alternative Names

SNCA; NACP; PARK1; PARK4; PD1; synuclein alpha

Gene ID

6622 (Human); 20617 (Mouse)

Uniprot ID

P37840

Product Pictures

FuncS

Figure 1 shows the results of passive immunotherapy with antibodies on memory performance in the probe portion of the Morris water maze test.

Pepscan analysis revealed that NI-202.21D11 overlapped with peptides B08 (aa 109-123 of SEQ ID NO: 1), B09 (aa 113-127 of SEQ ID NO: 1) and B10 (aa of SEQ ID NO: 117-131) Binding to human α-synuclein of 1) shows that the minimal sequence required for NI-202.21D11 binding is PVDPDNE within human α-synuclein (aa 117-123 of SEQ ID NO: 1).

ELISA

Figure 2 shows the results of an ELISA assay testing the affinity of different humanized 5C1 antibodies.

Figure 3 shows the results of an alanine scanning mutagenesis experiment used to determine the epitope of [alpha]-synuclein to which the 5D12 antibody binds.

The upper part of the panel shows a western blot of full-length α-synuclein (wild-type or a single point mutation in residues 118-126, as indicated) stained with the 5D12 antibody.

Publications