NeuroMab™ Anti-Amyloid Beta 1-15 Antibody(NRP-0422-P645)

Figure 1 is a graph depicting the binding of Aβ to chimeric 3D6 (PK1614) compared to mouse 3D6.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates in PDAPP mouse brain tissue. IgG is used as a negative control in this experiment because it cannot bind Aβ and therefore cannot induce phagocytosis.

Figure 2 is a graph depicting the competition of biotinylated 3D6 with unlabeled 3D6, PK1614 and 10D5 for Aβ binding.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates in PDAPP mouse brain tissue. IgG is used as a negative control in this experiment because it cannot bind Aβ and therefore cannot induce phagocytosis.

Figure 3 depicts the results of an ELISA measuring the binding of humanized 3D6v1 and chimeric 3D6 to aggregated Aβ.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates in PDAPP mouse brain tissue. IgG is used as a negative control in this experiment because it cannot bind Aβ and therefore cannot induce phagocytosis.

Figure 4 Immunohistochemistry of PDAPP brain sections showing the specificity of the h3D6v1 antibody.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates in PDAPP mouse brain tissue. IgG is used as a negative control in this experiment because it cannot bind Aβ and therefore cannot induce phagocytosis.

Figure 5 Competitive binding assay of h3D6.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates in PDAPP mouse brain tissue. IgG is used as a negative control in this experiment because it cannot bind Aβ and therefore cannot induce phagocytosis.

Figure 6 In vitro assay using h3D6v2 antibody. The ability of h3D6v2 to stimulate microglia was tested by an in vitro phagocytosis assay.

h3D6v2 was as effective as chimeric 3D6 in inducing phagocytosis of Aβ aggregates in PDAPP mouse brain tissue. IgG is used as a negative control in this experiment because it cannot bind Aβ and therefore cannot induce phagocytosis.