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NeuroMab™ Anti-PRNP BBB Shuttle Antibody,Clone NR1843P
- Host Species:
- Mouse
- Species Reactivity:
- Human
- Applications:
- IP; WB; ELISA; Inhib; In Vitro
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Notes: The BBB antibody is made-to order and available in a customized format. Please don't hesitate contact us for more details.
Species Reactivity
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Figure 3 Epitope mapping of the bovine PrP peptide library.
mAD 15B3 recognizes 3 distinct arrays of peptides
Figure 4. Immunoprecipitation of bovine, mouse and human PrP using mAbs 15B3 and 6H4
Supernatants from centrifuged homogenates of medulla from two different BSE-diagnosed animals or two normal animals were incubated with antibodies 6H4 or 15B3. Antibody was precipitated with Protein A (15B3) on Protein G-Sepharose (6H4).
Figure 5. Immunoprecipitation of bovine, mouse and human PrP using mAbs 15B3 and 6H4
Proteinase K digestion of immunoprecipitated PrP BSE using mA 15B3. Undigested and digested bovine brain homogenates were compared to immunoprecipitates digested with protein A-agarose only or proteinase K with 15B3.
Figure 6. Immunoprecipitation of bovine, mouse and human PrP using mAbs 15B3 and 6H4
Immunoprecipitation of mouse PrPSc using mAb 15B3. Homogenates from PrP-null mice (0/0) or wild type mice (normal (*/*) or scraping infection (+/+Sc)) were immunoprecipitated with mAb 15B3 or protein A-agarose only , and analyzed by Western blot
Figure 7. Immunoprecipitation of bovine, mouse and human PrP using mAbs 15B3 and 6H4
Immunoprecipitation of human PrP CJD using mAb 15B3. Brain homogenates (cerebellum) from normal individuals or patients with CJD type 1 (ref. 10) were immunoprecipitated and analyzed as described in a. Two representative examples from a total of 4 normal individuals and 4 CJD cases are shown.
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