NeuroMab™ Anti-PRNP BBB Shuttle Antibody, Clone 6H4

Figure 3 Epitope mapping of the bovine PrP peptide library.

mAD 15B3 recognizes 3 distinct arrays of peptides

Figure 4. Immunoprecipitation of bovine, mouse and human PrP using mAbs 15B3 and 6H4

Supernatants from centrifuged homogenates of medulla from two different BSE-diagnosed animals or two normal animals were incubated with antibodies 6H4 or 15B3. Antibody was precipitated with Protein A (15B3) on Protein G-Sepharose (6H4).

Figure 5. Immunoprecipitation of bovine, mouse and human PrP using mAbs 15B3 and 6H4

Proteinase K digestion of immunoprecipitated PrP BSE using mA 15B3. Undigested and digested bovine brain homogenates were compared to immunoprecipitates digested with protein A-agarose only or proteinase K with 15B3.

Figure 6. Immunoprecipitation of bovine, mouse and human PrP using mAbs 15B3 and 6H4

Immunoprecipitation of mouse PrPSc using mAb 15B3. Homogenates from PrP-null mice (0/0) or wild type mice (normal (*/*) or scraping infection (+/+Sc)) were immunoprecipitated with mAb 15B3 or protein A-agarose only , and analyzed by Western blot

Figure 7. Immunoprecipitation of bovine, mouse and human PrP using mAbs 15B3 and 6H4

Immunoprecipitation of human PrP CJD using mAb 15B3. Brain homogenates (cerebellum) from normal individuals or patients with CJD type 1 (ref. 10) were immunoprecipitated and analyzed as described in a. Two representative examples from a total of 4 normal individuals and 4 CJD cases are shown.