NeuroMab™ Mouse Anti-Human Connexin 43 Monoclonal Antibody (CBP2216)

Product Overview

Description

Connexins are a family of transmembrane proteins that assemble to form vertebrate gap junctions, thus also called Gap Junction Proteins. So far, 21 Connexins have been discovered in humans. The gene GJA1 encodes Connexin 43 (Cx43), which is the most expressed Connexin, and can be found in many human tissues including skin, bone, and brain. Cx43 has been reported to be involved in synchronized contraction of the heart as well as embryonic development. Besides its functions as a channel protein, Cx43 also performs channel-independent functions. During brain and heart development, cytoplasmic Cx43 regulates the microtubule network and thus affects cell migration and polarity. In addition, mitochondrial GJA1 has also been reported to promote cell survival by down-regulating the intrinsic apoptotic pathway during conditions such as oxidative stress

Immunogen

KLH-conjugated peptide corresponding to amino acids 247-382 of Connexin 43

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2216

Applications

WB; IHC-P

Application Notes

WB (1-5 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1642

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Connexin 43

Official Name

GJA1

Full Name

Gap junction alpha-1 protein

Alternative Names

GJA1; AVSD3; CMDR; CX43; EKVP; GJAL; HLHS1; HSS; ODDD; PPKCA; gap junction protein alpha 1; EKVP3

Gene ID

2697 (Human); 14609 (Mouse)

Uniprot ID

P17302 (Human); P23242 (Mouse)

References

1. Grek CL, et al. 2016. Cancer Lett. 374(1):117
2. Lilly E, et al. 2016. Semin Cell Dev Biol. 50:4
3. Michela P, et al. 2015. Eur J Pharmacol. 768:71

Publications