NeuroMab™ Mouse Anti-Human CD62E Monoclonal Antibody (CBP2314)

Product Overview

Description

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF-α, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162).The HAE-1f antibody has been shown to recognize human CD62E and to be useful for flow cytometry and in vitro blocking.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2314

Applications

Application Notes

FCM (≤1.0 µg per million cells in 100 µl volume)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1706

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD62E

Official Name

SELE

Full Name

E-selectin

Alternative Names

SELE; CD62E; ELAM; ELAM1; ESEL; LECAM2; selectin E

Gene ID

6401 (Human); 20339 (Mouse)

Uniprot ID

P16581 (Human); Q00690 (Mouse)

References

1. Collins T, et al. 1991. J. Biol. Chem. 266:2466
2. Bevilacqua MP, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9238
3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
4. Lawrence MB, et al. 1993. J. Immunol. 151:6338.
5. Walz G, et al. 1990. Science 250:1132

Publications