Single-Cell Electroporation Imaging

Single-cell electroporation Imaging has revolutionized the field of neuroscience by providing a powerful tool for studying the structure, function, and connectivity of individual neurons. By enabling the precise delivery of foreign molecules into neurons, this technique allows for the labeling, visualization, and manipulation of specific cells. Creative Biolabs is at the forefront of developing advanced single-cell electroporation imaging systems that enable researchers to explore the complexities of neural networks with unprecedented resolution and accuracy.

High-Throughput Single-Cell Electroporation Platform

Creative Biolabs has developed a state-of-the-art high-throughput single-cell electroporation system that significantly enhances the efficiency and speed of neuronal imaging experiments. This cutting-edge system enables simultaneous electroporation of multiple neurons, thereby increasing the throughput and accelerating data acquisition. The advanced technology incorporated into our system ensures precise and reliable delivery of exogenous molecules into target neurons, allowing for comprehensive and detailed investigations of neuronal properties.

Fig.1 Scalable single-cell electroporation platform for multiplexed genetic/protein delivery.¹

Advantages of Single-Cell Electroporation Imaging

• Cellular Specificity: Single-cell electroporation Imaging allows for the selective targeting of individual neurons, enabling researchers to study specific cell types or subpopulations within a complex neuronal network.
• Real-Time Monitoring: By incorporating fluorescent dyes or genetically encoded probes into neurons via electroporation, researchers can monitor neuronal activity in real-time. This capability enables the observation of dynamic changes in cellular properties, such as calcium signaling, action potential firing, and synaptic transmission.
• Non-invasiveness: Single-cell electroporation is a minimally invasive technique that causes minimal damage to the target neurons. The transient and reversible pores formed in the cell membrane during electroporation allow for the efficient delivery of molecules without compromising cell viability and functionality.
• Versatility: Creative Biolabs' single-cell electroporation Imaging is highly versatile, accommodating a wide range of experimental needs. It allows for the delivery of various molecules, including fluorescent dyes, genetically encoded sensors, optogenetic tools, and disease-associated genes, enabling diverse applications in neuroscience research.
• High-Throughput Capabilities: The offers high-throughput capabilities, allowing researchers to transfect multiple neurons simultaneously. This significantly accelerates experimentation timelines, enabling large-scale studies and screening of genetic libraries with improved efficiency.

Applicable Animal Models for Single-Cell Electroporation Imaging

Creative Biolabs' Single-Cell Electroporation Imaging can be applied to various animal models, including rodents (such as mice and rats), non-human primates, zebrafish, and Xenopus Laevis, to establish precise and reliable electroporation protocols tailored to specific research goals. These animal models serve as valuable tools for investigating neural development, circuitry, and disease mechanisms.

The combination of single-cell electroporation and live-cell imaging has opened up exciting possibilities for neuroscience research. Some key applications of this technology include:

• Neuronal Connectivity and Circuit Mapping
• Functional Characterization of Neuronal Subtypes
• Neurodevelopmental Studies
• Investigating Neurological Disorders

Creative Biolabs has been selected by our large pharmaceutical and biotechnology clients for our expertise in the area of neuroscience research. Our goal is to provide our customers with affordable services with reliable results.

Please feel free to contact us for more about our animal models related services.

Reference

1. Steinmeyer, Joseph D., and Mehmet Fatih Yanik. "High-throughput single-cell manipulation in brain tissue." PLoS One 7.4 (2012): e35603. Distributed under Open Access License CC BY 4.0 without modification.