Transgenic C. elegans Models for Aging
Aging is a complex biological process that affects all organisms, including humans. Understanding the molecular mechanisms underlying aging is crucial for developing interventions and treatments to promote healthy aging and prevent age-related diseases. At Creative Biolabs, we recognize the importance of reliable and efficient model systems for aging research. We have developed advanced transgenic C. elegans models specifically designed to investigate the intricate mechanisms of aging. Our innovative approach, coupled with our custom transgenic C. elegans model development service, enables us to provide researchers with invaluable tools to unravel the mysteries of aging.
Why Use C. elegans Models for Aging
In the nematode Caenorhabditis elegans, commonly known as C. elegans, approximately 60-80% of its nearly 20,000 genes are conserved with humans. The classic signaling pathway that influences aging, the insulin signaling pathway, was one of the first to be discovered in C. elegans. Other signaling pathways implicated in aging, such as the FOXO signaling pathway, TOR signaling pathway, sirtuin signaling pathway, and dietary restriction, are also conserved in humans. Additionally, C. elegans has a short lifespan, typically only 2-3 weeks under normal laboratory conditions, making it well-suited for studying aging phenotypes and mechanisms.
Fig. 1. Selected lifespan regulatory pathways in C. elegans. (Mack, 2018)
Our Approach
Our state-of-the-art transgenic technology allows for precise and efficient gene manipulation in C. elegans. We employ techniques such as microinjection, CRISPR/Cas9 genome editing, and RNA interference (RNAi) to introduce transgenes, knock out or knock down specific genes, and modulate gene expression levels. By utilizing the latest advancements in genetic engineering, we ensure the generation of robust and reliable transgenic C. elegans models for aging research.
Fig 2. The CRISPR-Cas9 genome editing approach in C. elegans. (Kim, 2022)
What We Can Provide?
1) Evaluation of the Effects of Genes or Drugs on C. elegans Lifespan:
We offer comprehensive assessments to determine the impact of genes or drugs on the lifespan of C. elegans. Through meticulous experimentation and analysis, we aim to identify potential anti-aging genes or drugs that can extend the lifespan of these organisms.
2) Mechanistic Studies of Gene or Drug Effects Using Genetic Approaches:
Our expertise extends to investigating the mechanisms underlying the effects induced by genes or drugs. We employ genetic techniques to delve into crucial signaling pathways related to aging, such as the FOXO signaling pathway, insulin-like signaling, reproductive signaling, dietary restriction signaling, and TOR signaling. By deciphering these mechanisms, we gain valuable insights into the aging process and potential targets for intervention.
3) Assessment of Key Parameters in C. elegans:
We offer comprehensive evaluations of various critical parameters in C. elegans to provide a comprehensive understanding of aging processes. These assessments include measuring free radical levels, muscle degeneration, gene expression levels, daf-16 nuclear localization, and locomotion ability. By quantifying these parameters, we gain valuable information about the impact of genes or drugs on aging-related phenotypes in C. elegans.
Our Custom Transgenic C. elegans Model Development Service
At Creative Biolabs, we understand that each research project is unique and may require tailored model systems to address specific scientific questions. To meet these diverse needs, we offer a custom transgenic C. elegans model development service.
Gene Design and Vector Construction
Creative Biolabs' team of experts employs state-of-the-art gene design strategies to construct vectors tailored to express AD-related genes or mutations in C. elegans. By utilizing precise genetic engineering techniques, they can generate transgenes with specific regulatory elements and control the expression of target genes in a tissue- or cell-specific manner.
Microinjection and Transgenic Line Generation
The transgenic C. elegans models are developed through microinjection techniques, which involve the precise introduction of transgenes into the nematode's germline. Creative Biolabs utilizes their cutting-edge facilities and equipment to efficiently generate stable transgenic lines. These lines can be propagated and maintained for long-term studies.
Phenotypic Analysis and Functional Studies
To assess the effectiveness of the custom transgenic C. elegans models, Creative Biolabs offers a comprehensive range of phenotypic analysis services. These include locomotion assays, lifespan analysis, behavioral studies, and electrophysiological measurements. These analyses enable the characterization of disease-related phenotypes.
We are committed to providing our clients with high-quality, reliable, and cost-effective Transgenic C. elegans Models for aging research. Please feel free to contact us for more about our C.elegans models related services.
References
- Mack, Hildegard I.D. et al. "The nematode Caenorhabditis elegans as a model for aging research." Drug Discovery Today: Disease Models 27 (2018): 3-13.
- Kim H-M, Hong Y, Chen J. A Decade of CRISPR-Cas Gnome Editing in C. elegans. International Journal of Molecular Sciences. 2022; 23(24):15863. https://doi.org/10.3390/ijms232415863
