Neurotransmitter Lipidoid-Doped Lipid Nanoparticle Technology

Central nervous system (CNS) diseases, such as Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS) and multiple sclerosis ( MS), etc., are the main challenges facing public health. There is an urgent need for researchers to attach great importance to protecting people from it. In the past few decades, different treatment strategies have been adopted, but their therapeutic effects are not enough and only partially relieve symptoms. The blood-brain barrier (BBB) has led to the main challenge for delivering drugs into the CNS to treat CNS disorders. The latest developments in nanotechnology provide opportunities to overcome the above-mentioned challenges raised by the BBB.

At Creative Biolabs, we provide a comprehensive list of neurotransmitter-derived lipid (NT-Lipidoid) doped lipid nanoparticles (LNP) to effectively facilited the delivery of BBB-impermeable cargos. The NT-Lipidoid LNP has been proven to help drug carrier crossing the blood-brain barrier, and further deliver the drug cargo to nerve cells. Such brain-delivered lipid tools can be used for studying brain function and pose great potential in the research of CNS disorders.

Design of NT Lipidoid-Doped LNP

NT is an endogenous chemical substance that can promote neurotransmission. Some NTs (e.g. dimethyltryptamine and tryptamine derivatives) have been demonstrated and are able to cross the BBB. To obtain LNP doped with NT lipids, we developed a list of lipidated NT derivatives, named "NT lipids", which can carry fluorescent proteins to efficiently pass through the BBB without the help of targeting ligands. NT1 lipids can be incorporated into lipid formulations that are otherwise impermeable to the BBB, and the resulting lipid nanoparticles (NT1-LNP) also have the ability to pass through the BBB.

Fig.1 Design of NT-lipidoids for effective brain delivery.

Validated Applications of NT Lipidoid-Doped LNP

Using LNP with NT lipids, researchers have successfully delivered a variety of drugs to the brains of mice:

• A small molecule antifungal drug, Amphotericin B;
• A macromolecule containing Tau antisense oligonucleotides that can inhibit the production of tau protein associated with Alzheimer's disease;
• Gene editing protein GFP-Cre.

With more than a decade of experience, Creative Biolabs provides an end-to-end solution from initial NP design, careful execution, accurate data description to timely result delivery. Our advanced technology, rich expertise and experienced scientists enable us to provide customers with the most valuable solutions to maximize the success rate of the project. If you are interested in our services, please send an email to contact us, and our team will get back to you as soon as possible.