Recombinase Tools

Creative Biolabs' recombinase tools are developed using the Cre-LoxP system strategy and can perform knockout, insertion, activation, inversion and translocation at specific sites on DNA. Our recombinase tools are highly efficient and temporally specific, have been validated in both eukaryotes and prokaryotes, and are functional in a variety of tissues and physiological settings. Tissue-specific or inducible promoters can be used to tightly regulate recombinase expression. Recombinase AAVs are excellent tools for a wide range of applications, including gene function research in neurological diseases and gene therapy.

Applications of the Cre-loxP System

Specific Labeling / Neural Circuit Tracing

Researchers can utilize Cre-strain mice and Cre-dependent viral vectors to mark and modify specific cells. The purpose of specific neural circuit labeling and functional research can be achieved by combining the Cre recombinase system with AAV serotypes (rAAV2/9, rAAV2/retro, and rAAV2/1) when using neural tracing technology to study the composition and function of specific neural circuits in the brain.

Virus-dependent Gene Recombination

While genetic recombination of transgenic mice is time-consuming, costly, and not highly specific to regions or tissues, an increasing number of researchers are turning to the Cre-loxP system for more flexibility. A common way is to introduce Cre or loxP elements using viruses and then combine them with a transgenic mouse.

Virus-dependent gene recombination provides the following advantages: It can achieve more robust regional and cell-specific gene recombination. The costs of virus preparation, storage, and injection are relatively modest. By delivering viruses to transgenic mice, the experimental time can be significantly reduced.

Other Recombinase Systems

System	Description
Flp-FRT	The Flp-FRT system, like the Cre-loxP system, induces gene recombination. The most notable distinction between the two systems is that the recombinases (Cre and Flp) have different optimal reaction temperatures. The ideal temperature for Cre recombinase is 37°C, while Flp recombinase is 30°C.
Dre-Rox	Dre (D6 site-specific DNA recombinase) is a tyrosine recombinase discovered in bacteriophages. It recognizes the 32-bp DNA sequence rox, which consists of two 14-bp reverse palindromic sequences and a 4-bp intermediate spacer region. Specific recombinases include Dre and Cre. Dre recombinase cannot recognize loxp sites, whereas Cre recombinase cannot detect rox sites.
vCre-vloxp / sCre-sloxp	vCre and sCre are both Cre recombinase homologs, however their homology is relatively low. Vloxp can be recognized especially by vCre. Sloxp can be recognized especially by sCre.

Please browse through our premade viruses list of recombinase tools to find the right recombinase tools for your experiment..

We offer a wide range of premade recombinase AAV particles to meet your needs. If you have any particular requirements or need help from our experienced staff, please do not hesitate to contact us so that we can accelerate your research.

Intellectual Property

Creative Biolabs is a contract manufacturer that produces tailored viruses and vectors according to client specifications. Importantly, this manufacturing service does not grant Creative Biolabs any intellectual property ownership; all designs and associated intellectual property rights remain exclusively with the customer.

Certain vector components may be subject to third-party intellectual property rights. Creative Biolabs offers publicly available sequence information for these components as a convenience for reference only. The customer assumes full responsibility and liability for the use of these components in their custom vectors. By purchasing vectors containing such components, the customer confirms they have secured all necessary intellectual property licenses, including the right to engage Creative Biolabs for manufacturing.

For Research Use Only. Not For Clinical Use.

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