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Creative Biolabs

Nthy-Ori 3-1 Human Thyroid Follicular Epithelial Cells

[CAT#: NCL-2108P51] Review(5) Q&As(3)

Species:
Human
Applications:
Cell Culture
Cell Types:
Other Cells

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Product Overview

Description

Transfect normal human primary thyroid follicular epithelial cells with a plasmid containing the defective SV40 genome (SV-ori) to make them immortal. SV40 DNA can immortalize normal thyroid epithelial cells while retaining several different functions.

Cell Types

Other Cells

Applications

Cell Culture

Species

Human
Properties

Size

>1x10^6 Cells

Growth Pattern

Adherent

Morphology

epithelioid

Culture Medium

RPMI-1640 + 10% FBS + 1% P/S

Freeze Medium

90%FBS+10%DMSO

Tissue Source

Thyroid

Shipping

Dry ice.

Storage

Liquid Nitrogen

Handling Advice

Passage ratio:1:2-1:4
Changing cell medium :2~3 times/week

Research Use Only

For research use only

Quality Control

• Each vial contains ≥ 1X10^6 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C, HPV, Herpes and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
Donor Information

Gender

Female

Donor Age

35 years old
Publications

Publications (0)

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Scientific Resources
Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
These cells have provided a robust model for our experiments
The cells arrive in excellent condition, and the viability is always above 90%. I appreciate the clear instructions for handling and the exceptional customer support. These cells have greatly advanced my research, allowing for reproducible and accurate data collection.
user
Excellent
Their performance in both short-term and long-term experiments has been excellent
They exhibit expected morphology and functional responses, making them ideal for in vitro studies. The documentation provided is thorough, facilitating smooth integration into our existing protocols.
user
Excellent
They have consistently met our expectations
As a researcher focused on thyroid autoimmunity, the Nthy-Ori 3-1 cells have provided a reliable platform for my studies. The cells’ response to cytokine treatment has been consistent, which is vital for my assays. The customer service team has been very helpful in addressing any questions I had about culturing conditions and handling.
user
Excellent
We’ve had great success with them
The cells’ growth rate is consistent, and they respond well to various experimental treatments. We’ve been able to generate significant data, thanks to the reliability of these cells. The ordering process is straightforward, and delivery is always prompt.
user
Excellent
Nthy-Ori 3-1 cells have provided a stable and reliable model
They exhibit the expected epithelial morphology and respond appropriately to stimuli. The supplier’s quality control ensures that each shipment of cells is of high quality, allowing for consistent experimental outcomes.
Q&As
Are Nthy-Ori 3-1 cells tumorigenic?
No, Nthy-Ori 3-1 cells are not tumorigenic. They are derived from normal human thyroid tissue and have been immortalized to facilitate long-term studies. However, they retain many characteristics of normal thyroid cells, making them suitable for comparative studies in thyroid cancer research, but they do not form tumors when implanted in vivo.
What biosafety level is required for handling Nthy-Ori 3-1 cells?
Nthy-Ori 3-1 cells should be handled in a laboratory equipped for Biosafety Level 1 (BSL-1) procedures. While they are derived from human tissue, they are not known to be infectious or pose a high risk to laboratory personnel. Standard aseptic techniques and personal protective equipment should be used to ensure safety.
How do I verify the authenticity of my Nthy-Ori 3-1 cell line?
To verify the authenticity of Nthy-Ori 3-1 cells, you can perform STR (short tandem repeat) profiling, which provides a genetic fingerprint of the cell line. Additionally, checking the expression of thyroid-specific markers and comparing them to established profiles can further confirm the cell line's identity. Regular testing ensures that your cell line remains uncontaminated and authentic.
For Research Use Only. Not For Clinical Use.
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