Pain Modeling and Drug Efficacy Testing Service

Disease Mechanism

Pain is an unpleasant sensation caused by actual or potential tissue damage and an early warning mechanism to prevent compression or further damage to the body. Neuropathic pain (NP) refers to pain caused by injury to the peripheral or central nervous system caused by trauma or disease. The primary injury is often healed without visible tissue damage. Central nervous system pain models include heavy object fall or contusion model, photochemical-induced spinal cord injury model, etc.; peripheral nerve injury models include neuroma model, chronic constriction injury model, partial sciatic nerve ligation model, spinal nerve ligation model, and tail nerve resection model, the sciatic neuritis model, etc. Disease-induced peripheral neuropathy models include herpes zoster nerve models, diabetic neuralgia models, etc.; in addition, there are cancer pain models.

Pain Modeling

1) Studies have found that injections of NMDA receptor antagonist drugs in humans can cause behavioral manifestations similar to Pain symptoms, including hallucinations, delusions, and bizarre behavior. Likewise, injections of such drugs in rodents have shown psychotic-like behavioral changes, such as sensorimotor gating disorders, hyperactivity, social withdrawal, and learning and memory dysfunction. Representative drugs of NMDA receptor antagonists include ketamine, PCP, and MIA-801.

2) Using a series of methods to damage some special brain areas of neonatal rats, such as the prefrontal cortex, hippocampus, and amygdala, because these brain areas play an important role in cognition and emotion in the brain, the damage to these areas simulates the mental state to some extent. Manifestations of Pain. The earliest study is the ventral hippocampus of neonatal rats. The injury model destroys the extensive cortical and subcortical neural circuits involved in the hippocampus, resulting in Pain-like manifestations. At present, this model is quite mature. The most rapidly developing model is the excitotoxic injury model of the basolateral amygdala in neonatal rat. This model has increased movement, increased auditory startle response, deficits in prepulse inhibition (PPI) of the startle reflex, and sustained latent inhibition. (LI) Abnormal, cognitive decline such as spatial learning ability and memory.

Assays after Modeling

The vorl Frey method is used to detect allodynia

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