AD Modeling and Drug Efficacy Testing Service
Disease Mechanism
Alzheimer's disease (AD), also known as senile dementia, is the most common type of senile dementia. The main manifestations are neuropsychiatric symptoms such as progressive memory impairment, cognitive dysfunction, personality changes, and language disorders. The etiology and pathogenesis of AD have not been elucidated. The characteristic pathological changes are extracellular senile plaques formed by β-amyloid deposition and intraneuronal neurofibrillary tangles formed by hyperphosphorylation of tau protein, neuronal loss with glial cell proliferation, etc.
AD Modeling
| Classification | Modeling Method | Animals |
|---|---|---|
| Alzheimer's disease model | Brain localization surgery | Rat |
| Surgical method Sterile 7mm electrodes from the dura mater and stimulate with 1mA DC | Rat | |
| Brain localization injection of amanitamin | Rat | |
| Brain-targeted injection of immunotoxin to eliminate basal forebrain cholinergic cells | Rat | |
| Intraperitoneal injection of scopolamine | Rat | |
| Injection of solution A into the hippocampus | Rat | |
| Stereotactic injection of okadaic acid in the brain | Rat | |
| Transgenic animals (APP, PS-1, PS-2, and apoE4, etc.) | Rat/Mouse | |
| Aluminum trichloride hexahydrate gavage (chronic aluminum poisoning) | Rat | |
| D-galactose (D-gal)-induced subacute aging model | Rat | |
| 24 months old (natural aging) | Rat |
Assays after Modeling
Creative Biolabs offers a variety of well-accepted behavioral tests to assess potential anxiolytic, antidepressant, or antipsychotic effects, as well as cognitively enhancing and disruptive effects on learning and memory (cognitive, psychiatric, neurological). We have developed a comprehensive range of rat primary neuronal culture models and assays to evaluate the neurotoxic, neuroprotective, or neurotrophic effects of test compounds (cellular models).
1) Cognitive function
2) Imunohistochemical detection (GFAP，NeuN，ChAT，APP，Aβ1-42，p-Tau, etc.), etc.
In addition to the above tests, we can also provide MRI/PET-CT in vivo imaging of small animals, cardiac ultrasound, X-ray films, tissue fluorescence imaging (whole brain slide scan, tissue immunofluorescence imaging), patch clamp on cells and brain slices, multi-channel in vivo electrophysiological recordings, respiration recordings, behavioral testing of pain, anxiety, depression, olfactory function-related (go/no-go) in awake animals.
Why Creative Biolabs?
With extensive research experience, Creative Biolabs also helps our clients validate and develop the most cost-effective experimental designs and protocols. According to your target indications, we can establish a screening program for your drug project. In-house expertise allows us to assist you in most CNS and PNS disorders. If you are interested in our service, please do not hesitate to contact us for more details.
