Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric), Mammalian

Description

Endothelin converting enzyme 1 (ECE1; EC: 3.4.24.71) is a membrane-bound metalloprotease that cleaves inactive large endothelin (big ET-1), which is the precursor of active endothelin. Studies have shown that large ET-1 can be used as a predictor of survival in patients with esophageal squamous cell carcinoma. ECE-1 is abundantly expressed in endothelial cells that produce mature ET-1 in vivo. Up to now, endothelin is the most effective vasoconstrictor known, with cytokine or hormone-like activity. Therefore, ECE-1 may play an important role in the pathogenesis of cardiovascular disease and Alzheimer's disease. Our Endothelin Converting Enzyme 1 Activity Assay Kit utilizes the ability of active ECE-1 to cleave synthetic substrates (MCA-based peptides) that release free fluorophores in the presence or absence of an ECE-1 inhibitor mixture. The released fluorophore can be easily quantified using a fluorescence microplate reader. Our kit uses a unique combination of substrate and inhibitor to specifically detect ECE-1 in a variety of biological samples. In the assay, the contribution of other enzymes with similar catalytic properties is compensated-namely endothelin converting enzyme 2 (ECE-2), angiotensin converting enzymes (ACE1, ACE2) and neprilysin. Our assay kit is simple, specific, and can detect ECE-1 activity as low as 0.5 µU.
Detection of ECE1 activity in tissue/cell lysates

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Fluorometric (Ex/Em = 320/420 nm)

Components

• ECE-1 Assay Buffer
• ECE-1 (lyophilized)
• ECE-1 Substrate (in DMSO)
• ECE-1 Inhibitor Mix
• MCA Standard (5 mM)

Shipping

Gel Pack

Storage

-20°C

Research Use Only

For research use only

Target

ECE1

Official Name

ECE1

Full Name

Endothelin converting enzyme 1

Alternative Names

ECE1, ECE, endothelin converting enzyme 1

Gene ID

1889(Human); 230857(Mouse)

Uniprot ID

P42892(Human); Q4PZA2(Mouse)