Product
Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric)
[CAT#: NK1120FY263]
Detection of ECE1 activity in tissue/cell lysates
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Endothelin converting enzyme 1 (ECE1; EC: 3.4.24.71) is a membrane-bound metalloprotease that cleaves inactive large endothelin (big ET-1), which is the precursor of active endothelin. Studies have shown that large ET-1 can be used as a predictor of survival in patients with esophageal squamous cell carcinoma. ECE-1 is abundantly expressed in endothelial cells that produce mature ET-1 in vivo. Up to now, endothelin is the most effective vasoconstrictor known, with cytokine or hormone-like activity. Therefore, ECE-1 may play an important role in the pathogenesis of cardiovascular disease and Alzheimer's disease. Genie's Endothelin Converting Enzyme 1 Activity Assay Kit utilizes the ability of active ECE-1 to cleave free fluorophore synthetic substrates (MCA-based peptides) in the presence or absence of ECE-1 inhibitor mixtures. The released fluorophore can be easily quantified using a fluorescence microplate reader. Our kit uses a unique combination of substrate and inhibitor to specifically detect ECE-1 in a variety of biological samples. In the assay, the contribution of other enzymes with similar catalytic properties is compensated-namely endothelin converting enzyme 2 (ECE-2), angiotensin converting enzymes (ACE1, ACE2) and neprilysin. Our assay kit is simple, specific, and can detect ECE-1 activity as low as 0.5 µU.
Species Reactivity
Mammalian
Relevant Diseases
Neurological Disorders
Properites
Detection Method
Fluorometric (Ex/Em = 320/420 nm)
Assay Type
Activity Assay Kit
Assay Time
Takes only 1-2 hrs
Components
ECE-1 Assay Buffer
ECE-1 (lyophilized)
ECE-1 Substrate (in DMSO)
ECE-1 Inhibitor Mix
MCA Standard (5 mM)
ECE-1 (lyophilized)
ECE-1 Substrate (in DMSO)
ECE-1 Inhibitor Mix
MCA Standard (5 mM)
Preparation and Storage
Shipping
Gel Pack
Storage
-20°C
Handling Advices
The antibody solution should be gently mixed before use.
Research Use Only
For research use only
Target Details
Data
Figure 1 MCA Standard Curve.
Figure 2 Measurement of purified ECE-1 (5.5 ng) and ECE-2 (12 ng) activities. The kit can effectively discriminate ECE-1 activity from ECE-2 activity.
Figure 3 ECE-1 activity in Mouse Lung (1.1 µg protein) with or without Inhibitor Mix.
Figure 4 Measurement of ECE-1 Activity in Mouse Lung Extracts (1.1 µg protein), Mouse Heart Extracts (1.5 µg protein) and HeLa lysates (1.5 µg protein).
Figure 2 Measurement of purified ECE-1 (5.5 ng) and ECE-2 (12 ng) activities. The kit can effectively discriminate ECE-1 activity from ECE-2 activity.
Figure 3 ECE-1 activity in Mouse Lung (1.1 µg protein) with or without Inhibitor Mix.
Figure 4 Measurement of ECE-1 Activity in Mouse Lung Extracts (1.1 µg protein), Mouse Heart Extracts (1.5 µg protein) and HeLa lysates (1.5 µg protein).
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
