iNeu™ Human Motor Neuron Progenitors (Healthy)
[CAT#: NCL-2101-ZP34]
- Species:
- Human
- Applications:
- Cell Assay
- Cell Types:
- Motor Neuron
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Creative Biolabs Human iPSC-Derived Motor Neuron Progenitors are derived from a healthy male donor.
Creative Biolabs iPSCs were differentiated to motor neuron progenitors using a combination of small molecules to regulate multiple signalling pathways. Initial expansion of iPSC-Motor Neuron Progenitors is possible before the terminal differentiation to motor neurons.
Creative Biolabs Human iPSC-Derived Motor Neurons have been characterised by ICC and the motor neurons after 14 days in culture express HB9, LIM3, OLIG2 and TUJ1. After a further 14 days they express choline acetyltransferase (ChAT) and islet-1 (ISL1).
Creative Biolabs iPSCs were differentiated to motor neuron progenitors using a combination of small molecules to regulate multiple signalling pathways. Initial expansion of iPSC-Motor Neuron Progenitors is possible before the terminal differentiation to motor neurons.
Creative Biolabs Human iPSC-Derived Motor Neurons have been characterised by ICC and the motor neurons after 14 days in culture express HB9, LIM3, OLIG2 and TUJ1. After a further 14 days they express choline acetyltransferase (ChAT) and islet-1 (ISL1).
Cell Types
Motor Neuron
Applications
Cell Assay
Species
Human
Properties
Size
≥2 x 106 cells
Growth Pattern
Adherent
Tissue Source
Fibroblasts (74 yr Male)
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
vapour phase nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.
For Research Use Only. Not For Clinical Use.
