Mouse Anti-CT-1 Monoclonal Antibody (CBP2139)

Description

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has been shown to be a pleiotrophic cytokine exerting overlapping actions with other IL-6 family members on a variety of cell types. Human CT-1 encodes a 201 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. CT-1 is expressed in high levels in heart, skeletal muscle, liver, lung, and kidney. Lower level of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor β subunit. A third α receptor subunit has recently been implicated in the receptor complex.
Human CT-1 Antibody (2H3G9), mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified full length human recombinant CT-1 protein (Swiss Prot: Q16619).

Immunogen

Purified full length human recombinant CT-1 protein (Swiss Prot: Q16619)

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2139

Applications

ELISA; WB

Application Notes

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.

ELISA Capture: 0.5-10 μg/ml

ELISA Detection: 0.05-0.2 μg/ml
Recommended antibody pairing for sandwich immunoassay:






Western Blot: 0.5-1.0 μg/ml

Other Applications: user-optimized

Conjugation

Unconjugated

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification

Protein A affinity column

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Handling Advice

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

SLC6A8

Official Name

SLC6A8

Full Name

solute carrier family 6 (neurotransmitter transporter), member 8

Alternative Names

Solute carrier family 6 member 8; CCDS1; CT1; CRT; Creatine transporter 1; CRTR; CTR5; Sodium- and chloride-dependent creatine transporter 1

Gene ID

6535(Human);102857(Mouse)

Uniprot ID

P48029(Human);Q8VBW1(Mouse)