Mouse Anti-Human C3a Monoclonal Antibody (CBP3882)

[CAT#: NAB2012443LS]

Anti-C3a Monoclonal Antibody

Host Species:: Mouse

Species Reactivity:: Human

Applications:: ELISA; IP; WB; Neut

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Product Overview

Description

C3, complement component 3, is a 195 kD glycoprotein heterodimer that is linked by disulfide bonds. It is the fourth complement component to react in the classical pathway and it is also a reactant in the alternative complement pathway. C3 convertase splits C3 into C3b and C3a. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Macrophages and neutrophils have receptors for C3b. C3a is released into the surrounding fluids. It can bind to receptors on basophils and mast cells triggering them to release their vasoactive contents (e.g., histamine). Because of the role of these materials in anaphylaxis, C3a is called an anaphylatoxin. In blood, the C3a fragment is rapidly desarginated by serum carboxypeptidase N enzyme to generate C3a desArg. Both C3a and C3a desArg are not glycosylated.

Immunogen

Human C3a

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP3882

Applications

ELISA; IP; WB; Neut

Application Notes

ELISA (2.0-6.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation; Neurodegeneration

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA.

Concentration

1.0 mg/mL

Purification

Affinity Chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

C3a

Official Name

C3a

Full Name

complement component 3a

Alternative Names

complement component 3a

References

1. de Bruijn MH, et al. 1985. P. Natl. Acad. Sci. USA 82:708.
2. Hugli TE, et al. 1975. J. Biol. Chem. 250:8293.
3. Oxvig C, et al. 1995. J. Biol. Chem. 270:13645.

Publications