Mouse HT-22 rho-0 Cell Line

Species:: Mouse

Cell Types:: Neurons

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Product Overview

Cell Types

Neurons

Application Notes

Grows slow. Split 1:3 twice a week, or as needed. Change medium 1-2 times a week. Do not grow to confluence.

Species

Mouse

Properties

Size

>1x10^6 Cells

Form

Frozen

Growth Pattern

Epithelial

Culture Medium

DMEM high glucose, 10% FBS, 1 mM pyruvate, 50 ug/ml uridine

Freeze Medium

Complete growth medium supplemented with 10% DMSO

Biosafety Level

BSL1

Tissue Source

Brain/hippocampus

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Cells are sensitive to acidification of the medium.

Research Use Only

This product is intended for research use only.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The cells are easy to culture

The cells exhibit predictable behavior, making them reliable for repeated experiments. Additionally, the documentation provided is thorough, ensuring smooth integration into our workflows. I highly recommend this cell line to anyone in the field.

Excellent

This cell line is a staple in our lab now

Our lab has been using the HT-22 rho-0 cell line for various experiments, and it has never disappointed us. The cells are robust, and we have not encountered any contamination issues. The technical support team is knowledgeable and always ready to help with troubleshooting.

Excellent

We have used the HT-22 rho-0 cell line for drug toxicity studies

It has proven to be highly effective. The cells' consistent response to various compounds has improved the reliability of our assays. The product quality is excellent, and the support team is knowledgeable and responsive. This cell line has become a critical component of our research.

Excellent

Highly recommended for neurotoxicity research

Using the Mouse HT-22 rho-0 cell line for our glutamate-induced toxicity studies has been highly effective. These cells show clear and consistent responses to glutamate exposure, allowing us to accurately measure neuronal cell death and stress responses. The cells are easy to culture and maintain, and their mitochondrial DNA-depleted status is ideal for isolating the effects of glutamate toxicity.

Excellent

Our lab focuses on neurodegenerative diseases, and the HT-22 rho-0 cell line has been a valuable tool in our research

The cells' response to glutamate toxicity is both predictable and measurable, allowing us to draw meaningful conclusions from our experiments. The technical support team has been very responsive and helpful in addressing our questions.

Q&As

What are the specific markers or endpoints that can be measured when studying glutamate-induced toxicity in the mouse HT-22 rho-0 cell line?

Researchers typically measure cell viability, mitochondrial membrane potential, reactive oxygen species production, and apoptosis markers in the cell line under glutamate-induced toxicity conditions. These endpoints provide insights into cellular responses to excitotoxic insults and potential neuroprotective mechanisms.

What are the limitations or considerations when using the cell line to study glutamate-induced toxicity?

Researchers should consider the specific metabolic alterations and mitochondrial dysfunction inherent in the mouse HT-22 rho-0 cell line, which may affect cellular responses to glutamate toxicity. Understanding these limitations is crucial for interpreting experimental results accurately and designing relevant controls.

How reproducible are the glutamate-induced toxicity assays using the cell line?

Glutamate-induced toxicity assays with the cell line demonstrate high reproducibility when standardized culture conditions and assay protocols are followed. Researchers can reliably quantify cell viability, mitochondrial function, and oxidative stress markers to assess neuroprotective effects of compounds.