Mouse Oligodendrocyte Precursor Cells

[CAT#: NRZP-1122-ZP248] Review(5) Q&As(3)

Species:: Mouse

Cell Types:: Oligodendrocytes (OPC)

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Product Overview

Description

Mouse oligodendrocyte precursor cells are isolated from cerebral cortex tissue; the brain is divided into left and right hemispheres, and the cerebral cortex (gray matter) covers most of each cerebral hemisphere and is where neuronal cell bodies are concentrated. Inside is white matter made up of nerve fibers or myelin sheaths. Oligodendrocytes are distributed in the central nervous system. In silver-stained specimens, oligodendrocytes are smaller than astrocytes, and their protrusions are also smaller and less, in the shape of beads, so they are called oligodendrocytes. cells or oligodendrocytes. Oligodendrocytes are myelinating glial cells of the central nervous system (CNS) that develop through oligodendrocyte progenitor cells, pre-oligodendrocyte progenitor cells, immature and less mature cells. dendritic cells and other stages. Some scholars divide oligodendrocytes into three types according to their developmental degree and morphology. However, cell development is a continuous process, and there are no strict boundaries in the evolution of its morphology, expression products and functions, so its classification is relative. These three types are: type I oligodendrocytes, also known as pre-O2A (pre-O2A progenitor cell). The cells are round, with a smooth surface and a diameter of about 3 μm. When mixed culture in vitro, they grow in clusters on the surface of astrocytes. They have a strong potential for division and proliferation. Attachment molecule (polysialic acid-neural cell adhesion molecule, PSA-NCAM) and so on. Type II oligodendrocytes often have bipolar or tripolar protrusions, and very few are unipolar protrusions with a diameter of about 7 μm, which have a certain ability to divide and proliferate. When cultured in vitro, they are bipotent cells that can differentiate into oligodendrocytes and type II astrocytes, so they are also called oligodendrocyte-type II astrocyte progenitor cells. -2 astrocyte progenitor cells, O2A). In addition to expressing GM1 and vimentin, O2A also expresses gangliosides GD3 and GQ (antibodies of GQ produced by lymphoma hybridoma strain A2B5), so A2B5 antibody is often used to label O2A. Type III oligodendrocytes no longer have the ability to divide and proliferate, and they are end-stage cells. The diameter is about 10 μm. According to their ability to form myelin sheaths, oligodendrocytes are divided into two categories: immature and mature. Immature OL cell bodies often protrude 4 to 5 relatively thick protrusions, and there are still A2B5 markers on the surface. At the same time, they also express O1-O4 antigens and have no ability to form myelin sheaths. Mature oligodendrocyte protrusions are like spider webs, and they express a large amount of galactocerebroside (GC), proteio lipid protein (PLP), myelin basic protein (MBP), etc. , has the ability to myelinize axons. In vitro cultured oligodendrocyte precursor cells (referred to as oligodendrocyte precursor cells) include pre-O2A and O2A and immature oligodendrocytes, the former two having the ability to proliferate.

Cell Types

Oligodendrocytes (OPC)

Application Notes

The in vitro culture period of primary cells is limited. It is recommended to use the special growth medium provided by Creative Biolabs and the correct handeling method to culture the cells, so as to ensure the best culture state of the cells.

Passage Number

2-3

Species

Mouse

Properties

Size

5×10^5 cells

Growth Pattern

Adherent

Morphology

Polygonal

Culture Medium

Contains FBS, growth factor supplements, Penicillin, Streptomycin, etc.

Tissue Source

brain tissue

Shipping

Dry ice

Storage

Frozen cells should be stored in liquid (vapor phase).

Research Use Only

This product is for research use only. It is not approved for use in humans or for therapeutic or diagnostic use.

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

They have been incredibly reliable

The cells arrived well-packaged and viable, with a high purity rate. Their responsiveness to differentiation protocols was excellent, making my experiments run smoothly. The customer service was also very supportive, helping me with any queries I had regarding the product.

Excellent

Greatly enhanced my research on myelination processes

The cells were easy to culture and showed consistent growth patterns. I appreciate the detailed instructions and the quality of the product. I’ve observed robust differentiation and the markers expressed were consistent with oligodendrocyte lineage, which was crucial for my experiments.

Excellent

The cells are of high quality and differentiate into mature oligodendrocytes efficiently

The packaging was secure, and the cells arrived in perfect condition. The technical support team was very helpful in guiding me through the initial setup. I highly recommend these cells for anyone studying oligodendrocyte biology.

Excellent

These are by far the best

They were easy to thaw and culture, and I achieved high differentiation efficiency in my assays. The company’s customer service was also excellent, providing prompt responses to my questions.

Excellent

I am extremely satisfied with the cells

They arrived quickly and in great condition, ready for immediate use. The differentiation protocols provided were very effective, and I achieved consistent results across multiple experiments. The cells exhibited high purity, which minimized experimental variability.

Q&As

What is the typical lifespan of mouse oligodendrocyte precursor cells in culture?

Mouse OPCs can generally be cultured for several weeks, depending on the conditions and passage number. However, their lifespan can vary based on factors such as media composition, cell density, and handling practices. It is essential to follow recommended protocols and monitor cell health regularly to ensure optimal performance and reproducibility of experiments.

Can these cells be genetically modified, and if so, what techniques are recommended?

Yes, Mouse OPCs can be genetically modified using techniques such as transfection or viral transduction. Common methods include using lentiviral or retroviral vectors to introduce genetic material. Researchers should optimize the transfection conditions and validate the expression of the introduced genes to ensure successful modification and functionality in subsequent experiments.

Are the cells provided with a specific growth medium or supplements?

Yes, the cells are typically provided with a recommended growth medium that includes essential nutrients and supplements necessary for their proliferation and maintenance.We may also provide additional growth factors or instructions for supplementing the medium to optimize cell growth and differentiation, depending on the specific research needs.