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Creative Biolabs

Rat F98 Cells

[CAT#: NCB2108FY0055] Review(5) Q&As(3)

Species:
Rat
Applications:
3D Cell Culture
Cell Types:
Tumor Cells

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Product Overview

Cell Types

Tumor Cells

Applications

3D Cell Culture

Application Notes

The F98 and RG2 gliomas can be used as rat brain tumor models in experimental neuro-oncology. This cell line may be used for both in vitro and in vivo studies of a rat brain tumor.

Relevant Diseases

Glioma

Research Areas

Neural Cell

Species

Rat
Properties

Form

Frozen

Growth Pattern

Adherent

Morphology

Glial

Culture Medium

The base medium for this cell line is Dulbecco's modified Eagle's medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Freeze Medium

Complete growth medium supplemented with 5% (v/v) DMSO

Tissue Source

Brain

Age

20 days gestation

Shipping

Dry ice

Storage

Vapor phase of liquid nitrogen

Handling Advice

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Research Use Only

For research use only
Donor Information

Donor Health

Tumor

Additional Donor Information

This tumor was produced by Drs. A. Koestner and W. Wechsler at Ohio State University in 1971.
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Publications (0)

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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
The cells were stable and showed consistent fluorescence
The fluorometric signals remained robust and reliable even in the complex 3D matrices we used. This stability allowed us to accurately measure cell viability and proliferation over extended periods.
user
Excellent
Using rat F98 cells in our 3D cell culture assays was a success
We incorporated rat F98 cells into our 3D glioma models, and the fluorometric readouts were consistent throughout the experiments. This consistency is crucial for studying tumor dynamics in a more physiologically relevant environment. The cells adapted well to 3D conditions.
user
Excellent
Worked well
Using rat F98 cells in 3D culture provided clear and reproducible fluorometric data. We were able to evaluate drug responses with high accuracy, thanks to the cells’ excellent performance in a 3D setting. Their reliability significantly improved our drug screening process.
user
Excellent
Great for detailed biomedical studies
They maintained their integrity and fluorescence intensity over time, which is essential for studying neural tissue interactions in three dimensions. These cells are now a key component of our neural research toolkit.
user
Excellent
Their performance has been highly satisfactory
The fluorometric signals were strong and stable, allowing us to monitor tumor growth and response to treatments with precision. Their adaptability to 3D environments is a major advantage.
Q&As
What type of fluorometric assay is best for use with rat F98 cells?
Rat F98 cells work well with a variety of fluorometric assays, including viability, proliferation, and apoptosis assays. The choice of assay depends on your specific research goals. Commonly used assays include those that measure metabolic activity, DNA synthesis, or fluorescence-based viability indicators, all of which provide reliable data with these cells.
How should I prepare rat F98 cells for use in a 3D culture system?
Prepare rat F98 cells by first thawing them according to standard cell culture protocols. Ensure they are fully resuspended and equilibrated before seeding into your 3D culture system. It’s important to maintain appropriate culture conditions and monitor cell density to achieve optimal growth and fluorescence in the 3D matrix.
What troubleshooting steps should I take if the fluorometric signals from rat F98 cells are not as expected?
If fluorometric signals are not as expected, first check the cell viability and ensure they are properly acclimated to the 3D culture environment. Verify that the fluorometric assay reagents are fresh and correctly applied. Additionally, ensure that the culture conditions are consistent and that there are no issues with the equipment used for measurement.
For Research Use Only. Not For Clinical Use.
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