Thyrotropin-releasing Hormone Receptor Assay Cell Line
[CAT#: NCL2110P314]
- Cell Types:
- Other Cells
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Product Overview
Description
Cell line information: TRH1/U2OS
Official Full Name: Thyrotropin-releasing hormone receptor
DNA Accession Number: GenBank: AY493373
Host Cell: U2OS
Format: 2 cryopreserved vials
Resistance: Puromycin
Storage: Liquid Nitrogen
Official Full Name: Thyrotropin-releasing hormone receptor
DNA Accession Number: GenBank: AY493373
Host Cell: U2OS
Format: 2 cryopreserved vials
Resistance: Puromycin
Storage: Liquid Nitrogen
Features
The HiTector TRH1 contains U2OS cells stably expressing unlabeled human thyroid-stimulating hormone releasing hormone receptor (TRH1). The HiTector TRH1 cell line is designed to test compounds or analyze their ability to modulate the thyroid-stimulating hormone releasing hormone receptor. When the agonist binds to TRH1, it activates the G protein, which in turn triggers a cellular response mediated by the second messenger (calcium). This cell line has been validated by measuring the increase in calcium in the cytosol. The high reproducibility of this assay allows the TRH1 activation process to be monitored in high-throughput screening.
Cell Types
Other Cells
Research Areas
GPCR
Assay
Thyrotropin-releasing Hormone Receptor Assay
Assay Description
Thyroid-stimulating hormone-releasing hormone (TRH), also known as thyroid-stimulating hormone-releasing factor (TRF) or thyroid hormone, is one of the smallest hormones. The thyroid-stimulating hormone-releasing hormone receptor assay allows measurement of the activity and potency of recombinant human TRH.
To analyze the activity of rhTRH, we used a stable cell line expressing thyroid stimulating hormone releasing hormone receptor subtype 1 (TRH1). When rhTRH binds to TRH1, it activates the G protein, which in turn triggers a cellular response mediated by the second messenger (Ca2+). The high reproducibility of this assay allows monitoring of the activation process of the thyroid-stimulating hormone releasing hormone receptor in high-throughput screening.
To analyze the activity of rhTRH, we used a stable cell line expressing thyroid stimulating hormone releasing hormone receptor subtype 1 (TRH1). When rhTRH binds to TRH1, it activates the G protein, which in turn triggers a cellular response mediated by the second messenger (Ca2+). The high reproducibility of this assay allows monitoring of the activation process of the thyroid-stimulating hormone releasing hormone receptor in high-throughput screening.
Assay Characteristics
Readout: Calcium Flux
Agonist: rhTRH
EC50 Agonist: 1.26 × 106-6 mg/ml
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Agonist: rhTRH
EC50 Agonist: 1.26 × 106-6 mg/ml
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
TRHR
Full Name
Thyrotropin-Releasing Hormone Receptor
Alternative Names
TRHR; TRH-R; thyrotropin releasing hormone receptor; CHNG7
Publications
Publications (0)
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
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