Product

APP Processing Assay Cell Line

[CAT#: NCL2110P247]

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Product Overview

Description

Cell line information: tGFP-APP MDCK Cell Line (Alzheimer's disease in vitro model)

Features

tGFP-APP MDCK cells allow analysis to assess the endogenous secretase proteolytic process in living cells. This cell line has been validated with different inhibitory compounds and siRNA for BACE to block γ-secretase and β-secretase activities to reduce their processing. Each batch of division-suppressing cells is highly validated and shows the same reference compound response as the stable cell line. The high-content analysis of gamma and beta secretase activity is designed to be performed using an epi-fluorescence imaging system to acquire and analyze images and quantify fluorescent vesicles entering the cytoplasm. The results obtained during the verification of the assay indicate that the pharmacological inhibition of the secretase involved in AD is an effective strategy for drug screening, and these models are suitable for monitoring the disease process in vivo in a bioimaging system.

Relevant Diseases

Alzheimer's Disease; CNS

Assay

APP Processing Assay

Assay Description

Creative Biolabs has developed a new fluorescence-based detection method for screening APP Processing Assay inhibitors in the APP Processing pathway. This new detection method requires the use of MDCK cell lines that stably express green fluorescent APP. Treating this cell line with an inhibitor of Aβ peptide formation such as an inhibitor of secretase activity, APP appears to remain in the fluorescent spot. It occurs in a dose response manner, allowing quantification using imaging systems. This model can recognize β-secretase and γ-secretase inhibitors, as well as any other compounds that prevent the formation of Aβ peptides.

Assay Characteristics

Readout: Fluorescent tGFP-APP retained vesicles
Type of Assay: Cell-based / Functional
Detection Method: Image Analysis
Pathway: APP Processing - Aβ Secretion
Assessment: γ & β Secretase Activity Assessment
HCS Application: Fluorescent APP Vesicles Quantification
Material provided: stable Cell Line (2 vial of cells); Division Arrested cells (2 million cells/vial)

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.