Clear3D™ Brain Tissue Clearing Kit

[CAT#: NRZP-0822-ZP25] Review(5) Q&As(3)

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Product Overview

Description

The Clear3D™ Clearing Kit enables the immunostaining and clearing of different tissues (such as mouse brain hemispheres, cerebral organoids, and human tumors) for their subsequent three dimensional (3D) imaging analysis.

Properites

Components

100 mL Permeabilization Solution; 125 mL 10× Antibody Staining Solution; 100 mL Clearing Solution.

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Incredibly efficient

The protocol is straightforward, and the results are consistently high quality. The kit effectively clears tissue with minimal background noise, making it ideal for detailed imaging.

Excellent

It has significantly improved our data quality

We've used several clearing kits in the past, but this kit stands out for its efficiency and ease of use. The cleared brain samples are perfectly transparent, allowing for high-resolution imaging and better analysis.

Excellent

User-friendly

The kit is exceptional. It delivers on its promise of effective tissue clearing without the lengthy processing times. It’s a crucial tool for our 3D imaging studies.

Excellent

Fast and efficient

It clears tissue uniformly, and the protocol is simple enough for any lab member to follow. It’s now our go-to clearing kit.

Excellent

A reliable tissue clearing solution

The kit’s clearing solution is highly effective, providing excellent clarity and contrast for our experiments.

Q&As

How long does it typically take to clear brain tissue using the kit?

The kit typically requires 2-3 days for the complete clearing process, depending on the size and type of tissue. The protocol involves several steps, including fixation and dehydration, before the final clearing stage. The time may vary based on the specific tissue and the desired level of transparency. It’s crucial to follow the protocol closely for optimal results.

How do I know if the clearing process is complete and the tissue is fully transparent?

The clearing process is complete when the tissue appears uniformly transparent without any cloudiness or residual opacity. You can verify the transparency by inspecting the tissue under a light source or microscope. If needed, additional clearing time or solution changes may be required to achieve optimal results.

Can I reuse the reagents in the kit?

The reagents in the kit are intended for single-use to ensure the highest quality of results and to prevent contamination. Reusing reagents can lead to inconsistencies in the clearing process and may affect the clarity of the tissue. It’s best to use fresh reagents for each batch of samples.